ELISA/assay

Human Tenascin-c, TNC ELISA Kit

MBS703402

96 Strip Wells

850 USD

ELISA Kit

Human Tenascin-c, TNC ELISA Kit

troponin C type 1 (slow)

Human Tenascin-c (TNC) ELISA Kit; CMD1Z; TNC; TNNC; cardiac troponin C; slow twitch skeletal/cardiac muscle troponin C; troponin C; slow; troponin C1; slow; troponin C type 1 (slow)

troponin C, slow skeletal and cardiac muscles; Troponin C, slow skeletal and cardiac muscles; troponin C, slow skeletal and cardiac muscles; troponin C1, slow; cardiac troponin C; slow twitch skeletal/cardiac muscle troponin C; troponin C type 1 (slow)

TNNC1

TNNC1; TNNC1; TNC; TN-C; TNNC; CMD1Z; CMH13; TNNC; TN-C

TNNC1_HUMAN

Human

161

This assay has high sensitivity and excellent specificity for detection of human TNC. No significant cross-reactivity or interference between human TNC and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: For the quantitative determination of human tenascin-c (TNC) concentrations in serum, plasma and tissue homogenates. Detection Range: 0.78 ng/ml-50 ng/ml.

Sensitivity: The minimum detectable dose of human TNC is typically less than 0.195 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TNC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNC is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNC bound in the initial step. The color development is stopped and the intensity of the color is measured.

4507615

NP_003271.1

NM_003280.2

P63316

18,403 Da

Adrenergic Signaling In Cardiomyocytes Pathway 908257!!Adrenergic Signaling In Cardiomyocytes Pathway 909696!!Calcium Signaling Pathway 83050!!Calcium Signaling Pathway 459!!Cardiac Muscle Contraction Pathway 93344!!Cardiac Muscle Contraction Pathway 93992!!Dilated Cardiomyopathy Pathway 121494!!Dilated Cardiomyopathy Pathway 121285!!Hypertrophic Cardiomyopathy (HCM) Pathway 114229!!Hypertrophic Cardiomyopathy (HCM) Pathway 106591

Troponin is a central regulatory protein of striated muscle contraction, and together with tropomyosin, is located on the actin filament. Troponin consists of 3 subunits: TnI, which is the inhibitor of actomyosin ATPase; TnT, which contains the binding site for tropomyosin; and TnC, the protein encoded by this gene. The binding of calcium to TnC abolishes the inhibitory action of TnI, thus allowing the interaction of actin with myosin, the hydrolysis of ATP, and the generation of tension. Mutations in this gene are associated with cardiomyopathy dilated type 1Z. [provided by RefSeq, Oct 2008]

Function: Troponin is the central regulatory protein of striated muscle contraction. Tn consists of three components: Tn-I which is the inhibitor of actomyosin ATPase, Tn-T which contains the binding site for tropomyosin and Tn-C. The binding of calcium to Tn-C abolishes the inhibitory action of Tn on actin filaments.

96 Strip Wells

850 USD