ELISA/assay

Mouse pulmonary surfactant-associated protein D, SP-D ELISA Kit

MBS703398

48-Strip-Wells

510 USD

ELISA Kit

Mouse pulmonary surfactant-associated protein D, SP-D ELISA Kit

pulmonary surfactant-associated protein D (SP-D)

Mouse pulmonary surfactant-associated protein D (SP-D) ELISA kit; pulmonary surfactant-associated protein D (SP-D)

surfactant protein D; Pulmonary surfactant-associated protein D; pulmonary surfactant-associated protein D; collectin 7; collectin-7; lung surfactant protein D; pulmonary surfactant apoprotein; surfactant, pulmonary-associated protein D; surfactant-associated protein, pulmonary 4; surfactant protein D; Collectin-7; Lung surfactant protein D

SP-D

SFTPD; SFTPD; SP-D; PSP-D; SFTP4; COLEC7; COLEC7; PSPD; SFTP4; PSP-D; SP-D

SFTPD_HUMAN

Mouse

375

This assay has high sensitivity and excellent specificity for detection of mouse SP-D. No significant cross-reactivity or interference between mouse SP-D and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 1.56 ng/ml -100 ng/ml. Sensitivity: The minimum detectable dose of mouse SP-D is typically less than 0.39 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SP-D has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SP-D present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SP-D is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SP-D bound in the initial step. The color development is stopped and the intensity of the color is measured.

292507

AAB59450.1

P35247

37,728 Da

FOXA1 Transcription Factor Network Pathway (137979); Phagosome Pathway (153910); Phagosome Pathway (153859); Regulation Of Retinoblastoma Protein Pathway (137916)

The protein encoded by this gene is part of the innate immune response, protecting the lungs against inhaled microorganisms and chemicals. The encoded protein may also be involved in surfactant metabolism. [provided by RefSeq, Jul 2015]

Function: Contributes to the lung's defense against inhaled microorganisms, organic antigens and toxins. Interacts with compounds such as bacterial lipopolysaccharides, oligosaccharides and fatty acids and modulates leukocyte action in immune response. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties.1 PublicationManual assertion based on experiment in:Ref.9

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800