catalog number :
MBS703396
products type :
ELISA Kit
products full name :
Rabbit apolipoprotein B100, Apo-B100 ELISA Kit
products short name :
apolipoprotein B100 (Apo-B100)
products name syn :
Rabbit apolipoprotein B100 (Apo-B100) ELISA Kit; Rabbit Apolipoprotein B-100 (APOB)
other names :
Apolipoprotein B, partial; Apolipoprotein B
products gene name :
APOB
uniprot entry name :
APOB_RABIT
specificity :
This assay has high sensitivity and excellent specificity for detection of Rabbit Apo-B100. No significant cross-reactivity or interference between Rabbit Apo-B100 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, tissue homogenates. Detection Range: 78 ng/ml-5000 ng/ml. Detection Wavelength: 450 nm. Sensitivity: 19.5ng/ml
other info2 :
Sample Volume: 50-100ul. Protein Biological Process 1: Apolipoprotein
products description :
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Apo-B100. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for Apo-B100. The competitive inhibition reaction is launched between with pre-coated Apo-B100 and Apo-B100 in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of Apo-B100 in the samples. The color development is stopped and the intensity of the color is measured.
ncbi mol weight :
15,664 Da
ncbi pathways :
Cell Surface Interactions At The Vascular Wall Pathway 106062!!Chylomicron-mediated Lipid Transport Pathway 106157!!FOXA1 Transcription Factor Network Pathway 137979!!Fat Digestion And Absorption Pathway 194385!!Fat Digestion And Absorption Pathway 194324!!Hemostasis Pathway 106028!!LDL-mediated Lipid Transport Pathway 106159!!Lipid Digestion, Mobilization, And Transport Pathway 106111!!Lipoprotein Metabolism Pathway 106156!!Metabolism Pathway 477135
uniprot summary :
Function: Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.