ELISA/assay

Human H-Cadherin, H-Cad/CDH13 ELISA Kit

MBS703388

48-Strip-Wells

565 USD

ELISA Kit

Human H-Cadherin, H-Cad/CDH13 ELISA Kit

cadherin 13, H-cadherin (heart)

Human H-Cadherin; H-Cad/CDH13 ELISA Kit; CDHH; H-cadherin; P105; T-cad; T-cadherin; cadherin 13; heart-cadherin; truncated-cadherin; cadherin 13; H-cadherin (heart)

cadherin-13 isoform 3; Cadherin-13; cadherin-13; T-cad; T-cadherin; heart cadherin; H-cadherin (heart); cadherin 13, H-cadherin (heart); cadherin 13; Heart cadherin; H-cadherin; P105; Truncated cadherin; T-cad; T-cadherin

CDH13

CDH13; CDH13; CDHH; P105; CDHH; H-cadherin; T-cad; T-cadherin

CAD13_HUMAN

Human

674

This assay has high sensitivity and excellent specificity for detection of Human CDH13. No significant cross-reactivity or interference between Human CDH13 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma and tissue homogenates. Assay Type: Sandwich. Detection Range: 1.25 ng/ml-80 ng/ml. Sensitivity: 0.312 ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Cell Adhesion. Protein Biological Process 3: Cell adhesion

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for H-Cad/CDH13 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any H-Cad/CDH13 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for H-Cad/CDH13 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of H-Cad/CDH13 bound in the initial step. The color development is stopped and the intensity of the color is measured.

333944018

NP_001207418.1

NM_001220489.1

P55290

78,287 Da

Adherens Junctions Interactions Pathway (119533); Cell Junction Organization Pathway (160966); Cell-Cell Communication Pathway (477132); Cell-cell Junction Organization Pathway (119532)

This gene encodes a member of the cadherin superfamily. The encoded protein is localized to the surface of the cell membrane and is anchored by a GPI moiety, rather than by a transmembrane domain. The protein lacks the cytoplasmic domain characteristic of other cadherins, and so is not thought to be a cell-cell adhesion glycoprotein. This protein acts as a negative regulator of axon growth during neural differentiation. It also protects vascular endothelial cells from apoptosis due to oxidative stress, and is associated with resistance to atherosclerosis. The gene is hypermethylated in many types of cancer. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2011]

Function: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. May act as a negative regulator of neural cell growth.1 PublicationManual assertion based on experiment in:Ref.9

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150