catalog number :
MBS703387
products type :
ELISA Kit
products full name :
Rat soluble cluster of differentiation 14, sCD14 ELISA Kit
products short name :
CD14 molecule
products name syn :
Rat soluble cluster of differentiation 14; sCD14 ELISA Kit; CD14 antigen; monocyte differentiation antigen CD14; CD14 molecule
other names :
monocyte differentiation antigen CD14; Monocyte differentiation antigen CD14; monocyte differentiation antigen CD14; myeloid cell-specific leucine-rich glycoprotein; Myeloid cell-specific leucine-rich glycoprotein; CD_antigen: CD14
products gene name :
CD14
other gene names :
CD14; CD14
uniprot entry name :
CD14_RABIT
specificity :
This assay has high sensitivity and excellent specificity for detection of rat sCD14. No significant cross-reactivity or interference between rat sCD14 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 28 ng/ml -1800 ng/ml. Sensitivity: 7 ng/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for sCD14 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any sCD14 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for sCD14 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of sCD14 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001075664.1
ncbi gb acc num :
NM_001082195.2
ncbi mol weight :
39,992 Da
ncbi pathways :
Amoebiasis Pathway (167315); Amoebiasis Pathway (167191); Hematopoietic Cell Lineage Pathway (84317); Hematopoietic Cell Lineage Pathway (489); Legionellosis Pathway (469198); Legionellosis Pathway (469186); MAPK Signaling Pathway (198550); MAPK Signaling Pathway (84287); MAPK Signaling Pathway (456); NF-kappa B Signaling Pathway (634529)
uniprot summary :
Function: In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the MD-2/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules
size4 :
10x96-Strip-Wells