product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Rat soluble cluster of differentiation 14, sCD14 ELISA Kit
catalog :
MBS703387
quantity :
48-Strip-Wells
price :
510 USD
more info or order :
product information
catalog number :
MBS703387
products type :
ELISA Kit
products full name :
Rat soluble cluster of differentiation 14, sCD14 ELISA Kit
products short name :
CD14 molecule
products name syn :
Rat soluble cluster of differentiation 14; sCD14 ELISA Kit; CD14 antigen; monocyte differentiation antigen CD14; CD14 molecule
other names :
monocyte differentiation antigen CD14; Monocyte differentiation antigen CD14; monocyte differentiation antigen CD14; myeloid cell-specific leucine-rich glycoprotein; Myeloid cell-specific leucine-rich glycoprotein; CD_antigen: CD14
products gene name :
CD14
other gene names :
CD14; CD14
uniprot entry name :
CD14_RABIT
reactivity :
Rat
sequence length :
372
specificity :
This assay has high sensitivity and excellent specificity for detection of rat sCD14. No significant cross-reactivity or interference between rat sCD14 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 28 ng/ml -1800 ng/ml. Sensitivity: 7 ng/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for sCD14 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any sCD14 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for sCD14 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of sCD14 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
126722873
ncbi acc num :
NP_001075664.1
ncbi gb acc num :
NM_001082195.2
uniprot acc num :
Q28680
ncbi mol weight :
39,992 Da
ncbi pathways :
Amoebiasis Pathway (167315); Amoebiasis Pathway (167191); Hematopoietic Cell Lineage Pathway (84317); Hematopoietic Cell Lineage Pathway (489); Legionellosis Pathway (469198); Legionellosis Pathway (469186); MAPK Signaling Pathway (198550); MAPK Signaling Pathway (84287); MAPK Signaling Pathway (456); NF-kappa B Signaling Pathway (634529)
uniprot summary :
Function: In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the MD-2/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules
size1 :
48-Strip-Wells
price1 :
510 USD
size2 :
96-Strip-Wells
price2 :
725
size3 :
5x96-Strip-Wells
price3 :
2565
size4 :
10x96-Strip-Wells
price4 :
4800
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

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