catalog number :
MBS703385
products type :
ELISA Kit
products full name :
Human Metallothionein-2, MT-2 ELISA Kit
products short name :
metallothionein 2A
products name syn :
Human Metallothionein-2 (MT-2) ELISA Kit; MT2; ; metallothionein 2A
other names :
metallothionein-2; Metallothionein-2; metallothionein-2; MT-2; MT-II; metallothionein-2A; metallothionein-II; metallothionein 2A; Metallothionein-2A; Metallothionein-II; MT-II
products gene name :
MT2A
other gene names :
MT2A; MT2A; MT2; CES1; MT2; MT-2; MT-II
uniprot entry name :
MT2_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of Human MT2A. No significant cross-reactivity or interference between Human MT2A and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 0.312 ng/ml-20 ng/ml. Sensitivity: 0.078 ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MT-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MT-2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MT-2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MT-2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_005944.1
ncbi gb acc num :
NM_005953.3
ncbi mol weight :
6,042 Da
ncbi pathways :
Cytokine Signaling In Immune System Pathway (366171); Immune System Pathway (106386); Interferon Signaling Pathway (187103); Interferon Gamma Signaling Pathway (187106); Mineral Absorption Pathway (212237); Mineral Absorption Pathway (212220)
uniprot summary :
Function: Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.
size4 :
10x96-Strip-Wells