Rat peroxisome proliferators activator receptors alpha, PPAR-alpha ELISA Kit | MyBioSource MBS703375 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat peroxisome proliferators activator receptors alpha, PPAR-alpha ELISA Kit

catalog :

MBS703375

quantity :

96 Strip Wells

price :

575 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS703375

products type :

ELISA Kit

products full name :

Rat peroxisome proliferators activator receptors alpha, PPAR-alpha ELISA Kit

products short name :

peroxisome proliferator-activated receptor alpha

products name syn :

Rat peroxisome proliferators activator receptors alpha; PPAR-alpha ELISA Kit; MGC2237; MGC2452; NR1C1; PPAR; PPARalpha; hPPAR; OTTHUMP00000197740; OTTHUMP00000197741; peroxisome proliferative activated receptor; alpha; peroxisome proliferator-activated receptor alpha

other names :

peroxisome proliferator-activated receptor alpha; Peroxisome proliferator-activated receptor alpha; peroxisome proliferator-activated receptor alpha; PPAR-alpha; nuclear receptor subfamily 1 group C member 1; peroxisome proliferator activated receptor alpha; Nuclear receptor subfamily 1 group C member 1

products gene name :

PPARA

other gene names :

Ppara; Ppara; PPAR; Nr1c1; Ppar; PPAR-alpha

uniprot entry name :

PPARA_RAT

reactivity :

Rat

sequence length :

468

specificity :

This assay has high sensitivity and excellent specificity for detection of Rat PPARA. No significant cross-reactivity or interference between Rat PPARA and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, tissue homogenates and cell lysates. Detection Range: 0.312 ng/ml-20 ng/ml. Detection Wavelength: 450 nm. Sensitivity: 0.078 ng/ml

other info2 :

Sample Volume: 50-100ul. Protein Biological Process 1: Transcription/Transcription regulation. Protein Biological Process 3: Transcription

products description :

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PPAR-alpha. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for PPAR-alpha and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain PPAR-alpha, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of PPAR-alpha in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

6981382

ncbi acc num :

NP_037328.1

ncbi gb acc num :

NM_013196.1

uniprot acc num :

P37230

ncbi mol weight :

52,377 Da

ncbi pathways :

Activation Of Gene Expression By SREBF (SREBP) Pathway 1010720!!Adipocytokine Signaling Pathway 83485!!Adipocytokine Signaling Pathway 505!!Adipogenesis Pathway 198479!!BMAL1:CLOCK,NPAS2 Activates Circadian Gene Expression Pathway 1010371!!Circadian Clock Pathway 1010370!!Developmental Biology Pathway 1009870!!Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway 1010691!!Gene Expression Pathway 1010398!!Generic Transcription Pathway 1010399

ncbi summary :

nuclear steroid receptor; heterodimerizes with retinoid-X receptor (RXR), and binds to peroxisome proliferator response elements (PPREs) to initiate the transcriptional regulation of target genes [RGD, Feb 2006]

uniprot summary :

Function: Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety. Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. May be required for the propagation of clock information to metabolic pathways regulated by PER2

size :

96 Strip Wells

price :

575 USD

more info or order :

MyBioSource product webpage