ELISA/assay

Human Vascular Endothelial-Cadherin Complex, VE-cad ELISA Kit

MBS703368

96 Strip Wells

850 USD

ELISA Kit

Human Vascular Endothelial-Cadherin Complex, VE-cad ELISA Kit

cadherin 5, type 2 (vascular endothelium)

Human Vascular Endothelial-Cadherin Complex; VE-cad ELISA Kit; 7B4; CD144; FLJ17376; 7B4 antigen; VE-cadherin; cadherin 5; type 2; cadherin 5; type 2; VE-cadherin (vascular epithelium) ; cd144 antigen; endothelial-specific cadherin; vascular endothelial cadherin; cadherin 5; type 2 (vascular endothelium)

cadherin-5 preproprotein; Cadherin-5; cadherin-5; 7B4 antigen; VE-cadherin; cd144 antigen; endothelial-specific cadherin; vascular endothelial cadherin; cadherin 5, type 2, VE-cadherin (vascular epithelium); cadherin 5, type 2 (vascular endothelium); 7B4 antigen; Vascular endothelial cadherin; VE-cadherin; CD_antigen: CD144

CDH5

CDH5; CDH5; 7B4; CD144; VE-cadherin

CADH5_HUMAN

Human

784

This assay has high sensitivity and excellent specificity for detection of Human CDH5. No significant cross-reactivity or interference between Human CDH5 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, tissue homogenates. Detection Range: 3.9 ng/ml-250 ng/ml. Detection Wavelength: 450 nm. Sensitivity: 0.98 ng/ml

Sample Volume: 50-100ul. Protein Biological Process 1: Cell Adhesion. Protein Biological Process 3: Cell adhesion

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for VE-cad has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VE-cad present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VE-cad is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VE-cad bound in the initial step. The color development is stopped and the intensity of the color is measured.

166362713

NP_001786.2

NM_001795.3

P33151

87,528 Da

Adherens Junctions Interactions Pathway 119533!!Cell Adhesion Molecules (CAMs) Pathway 83069!!Cell Adhesion Molecules (CAMs) Pathway 480!!Cell Junction Organization Pathway 160966!!Cell-Cell Communication Pathway 477132!!Cell-cell Junction Organization Pathway 119532!!Leukocyte Transendothelial Migration Pathway 83083!!Leukocyte Transendothelial Migration Pathway 494!!S1P2 Pathway 138059!!Signal Transduction Pathway 477114

This gene is a classical cadherin from the cadherin superfamily and is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. An alternative splice variant has been described but its full length sequence has not been determined. [provided by RefSeq, Jul 2008]

Function: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. Acts in concert with KRIT1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction.2 PublicationsManual assertion based on experiment in:Ref.10

96 Strip Wells

850 USD