catalog number :
MBS703363
products type :
ELISA Kit
products full name :
Mouse tissue inhibitors of metalloproteinase 3, TIMP-3 ELISA Kit
products short name :
TIMP metallopeptidase inhibitor 3
products name syn :
Mouse tissue inhibitors of metalloproteinase 3; TIMP-3 ELISA kit; HSMRK222; K222; K222TA2; SFD; MIG-5 protein; tissue inhibitor of metalloproteinase 3; TIMP metallopeptidase inhibitor 3
other names :
metalloproteinase inhibitor 3; Metalloproteinase inhibitor 3; metalloproteinase inhibitor 3; tissue inhibitor of metalloproteinases 3; tissue inhibitor of metalloproteinase 3; Tissue inhibitor of metalloproteinases 3; TIMP-3
products gene name :
TIMP3
other gene names :
Timp3; Timp3; Timp-3; Sun; Timp-3; TIMP-3
uniprot entry name :
TIMP3_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of Mouse TIMP3. No significant cross-reactivity or interference between Mouse TIMP3 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 46.88 pg/ml-3000 pg/ml. Sensitivity: 11.72 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TIMP-3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TIMP-3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TIMP-3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TIMP-3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_035725.1
ncbi gb acc num :
NM_011595.2
ncbi mol weight :
24,182 Da
ncbi pathways :
Endochondral Ossification Pathway (198336); Matrix Metalloproteinases Pathway (198370); MicroRNAs In Cancer Pathway (852715); MicroRNAs In Cancer Pathway (852928); Proteoglycans In Cancer Pathway (782024); Proteoglycans In Cancer Pathway (782054)
uniprot summary :
Function: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli.
size4 :
10x96-Strip-Wells