catalog number :
MBS703351
products type :
ELISA Kit
products full name :
Pig insulin-like growth factors binding protein 3, IGFBP-3 ELISA Kit
products short name :
insulin-like growth factor binding protein 3
products name syn :
Pig insulin-like growth factors binding protein 3; IGFBP-3 ELISA Kit; tcag7.703; BP-53; IBP3; IGF-binding protein 3; acid stable subunit of the 140 K IGF complex; binding protein 29; binding protein 53; growth hormone-dependent binding protein; insulin-like growth factor binding protein 3
other names :
insulin-like growth factor-binding protein 3; Insulin-like growth factor-binding protein 3; insulin-like growth factor-binding protein 3; IBP-3; IGF-binding protein 3
products gene name :
IGFBP3
other gene names :
IGFBP3; IGFBP3; IGFBP-3; IBP-3; IGF-binding protein 3; IGFBP-3
uniprot entry name :
IBP3_PIG
specificity :
This assay has high sensitivity and excellent specificity for detection of pig IGFBP-3. No significant cross-reactivity or interference between pig IGFBP-3 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 78 ng/ml -5000 ng/ml. Sensitivity: 19.5 ng/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IGFBP-3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IGFBP-3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IGFBP-3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IGFBP-3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001005156.1
ncbi gb acc num :
NM_001005156.1
ncbi mol weight :
31,690 Da
ncbi pathways :
Transcriptional Misregulation In Cancer Pathway (523029); Transcriptional Misregulation In Cancer Pathway (522987); P53 Signaling Pathway (84467); P53 Signaling Pathway (465)
uniprot summary :
Function: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R.
size4 :
10x96-Strip-Wells