catalog number :
MBS703346
products type :
ELISA Kit
products full name :
rabbit follicle-stimulating hormone, FSH ELISA Kit
products short name :
follicle-stimulating hormone, FSH
products name syn :
rabbit follicle-stimulating hormone; FSH ELISA Kit; follicle-stimulating hormone beta subunit; follitropin; beta chain; FSHB; follicle-stimulating hormone; FSH
other names :
follitropin subunit beta; Follitropin subunit beta; follitropin subunit beta; FSH-B; FSH-beta; follitropin beta chain; follicle stimulating hormone beta; follicle-stimulating hormone beta subunit; Follicle-stimulating hormone beta subunit; FSH-B; FSH-beta; Follitropin beta chain
other gene names :
FSHB; FSHB; FSH-B; FSH-beta
uniprot entry name :
FSHB_RABIT
specificity :
This assay has high sensitivity and excellent specificity for detection of rabbit FSH. No significant cross-reactivity or interference between rabbit FSH and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 1.56 mIU/ml -100 mIU/ml. Sensitivity: The minimum detectable dose of rabbit FSH is typically less than 0.39 mIU/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FSH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FSH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FSH is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FSH bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001075640.1
ncbi gb acc num :
NM_001082171.2
ncbi mol weight :
14,588 Da
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
uniprot summary :
Function: Stimulates development of follicle and spermatogenesis in the reproductive organs
size4 :
10x96-Strip-Wells