ELISA/assay

Rat eosinophil Cationic protein, ECP ELISA Kit

MBS703318

96 Strip Wells

850 USD

ELISA Kit

Rat eosinophil Cationic protein, ECP ELISA Kit

ribonuclease, RNase A family, 3 (eosinophil cationic protein)

Rat eosinophil cationic protein; ECP ELISA Kit; ECP; RNS3; cytotoxic ribonuclease; eosinophil cationic protein; ribonuclease; RNase A family; 3 (eosinophil cationic protein)

eosinophil cationic protein; Eosinophil cationic protein; eosinophil cationic protein; EAR-11; RNase 3; ribonuclease 1; ribonuclease 3; ribonuclease 7; eosinophil-associated ribonuclease; eosinophil secondary granule ribonuclease 11; eosinophil-associated, ribonuclease A family, member 11; Eosinophil secondary granule ribonuclease 11; EAR-11; Ribonuclease 3; RNase 3

RNASE3

Ear11; Rnase3; R1; R7; EAR; ECP; Rnase3; Ear11; Rns3; ECP; EAR-11; RNase 3

ECP_RAT

Rat

155

This assay has high sensitivity and excellent specificity for detection of Rat RNASE3. No significant cross-reactivity or interference between Rat RNASE3 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma and tissue homogenates. Detection Range: 62.5 pg/ml-4000 pg/ml. Detection Wavelength: 450 nm. Sensitivity: 15.6 pg/ml

Sample Volume: 50-100ul

Introduction: Eosinophil cationic protein (ECP) is a small, basic protein found in the matrix of the eosinophil's large specific granule that has cytotoxic, helminthotoxic, and ribonuclease activity, and is a member of the ribonuclease multigene family. Human eosinophil granules contain several basic proteins including ECP, eosinophil-derived neurotoxin (EDN) and major basic protein (MBP). ECP and MBP are potent helminthotoxins while EDN is less so. Both ECP and EDN possess neurotoxic and ribonuclease activities. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ECP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for ECP and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain ECP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of ECP in the samples is then determined by comparing the O.D. of the samples to the standard curve

20302099

NP_620257.1

NM_138902.1

P70709

18,007 Da

Asthma Pathway 83120!!Asthma Pathway 532

cationic protein with similarity to human ECP; also has similarity to human eosinophil-derived neurotoxin and human ribonuclease k6 [RGD, Feb 2006]

Function: Cytotoxin and helminthotoxin with ribonuclease activity. Possesses a wide variety of biological activities

96 Strip Wells

850 USD