Human tryptophanyl-tRNA synthetase, WARS ELISA Kit | MyBioSource MBS703294 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human tryptophanyl-tRNA synthetase, WARS ELISA Kit

catalog :

MBS703294

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS703294

products type :

ELISA Kit

products full name :

Human tryptophanyl-tRNA synthetase, WARS ELISA Kit

products short name :

tryptophanyl-tRNA synthetase

products name syn :

Human tryptophanyl-tRNA synthetase; WARS ELISA Kit; GAMMA-2; IFI53; IFP53; interferon-induced protein 53; tryptophan tRNA ligase 1; cytoplasmic; tryptophanyl-tRNA synthetase

other names :

tryptophan--tRNA ligase, cytoplasmic isoform a; Tryptophan--tRNA ligase, cytoplasmic; tryptophan--tRNA ligase, cytoplasmic; hWRS; trpRS; interferon-induced protein 53; tryptophan tRNA ligase 1, cytoplasmic; tryptophanyl-tRNA synthetase; Interferon-induced protein 53; IFP53; Tryptophanyl-tRNA synthetase; TrpRS; hWRS

products gene name :

WARS

other gene names :

WARS; WARS; IFI53; IFP53; GAMMA-2; IFI53; WRS; IFP53; TrpRS; hWRS

uniprot entry name :

SYWC_HUMAN

reactivity :

Human

sequence length :

471

specificity :

This assay has high sensitivity and excellent specificity for detection of human WARS. No significant cross-reactivity or interference between human WARS and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, Cell lysates and tissue homogenates. Assay Type: Sandwich. Detection Range: 0.312 ng/ml-20 ng/ml. Sensitivity: The minimum detectable dose of human WARS is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for WARS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any WARS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WARS is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WARS bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

47419914

ncbi acc num :

NP_004175.2

ncbi gb acc num :

NM_004184.3

uniprot acc num :

P23381

ncbi mol weight :

53,165 Da

ncbi pathways :

Aminoacyl-tRNA Biosynthesis Pathway (83030); Aminoacyl-tRNA Biosynthesis Pathway (424); Aminoacyl-tRNA Biosynthesis, Eukaryotes Pathway (413439); Aminoacyl-tRNA Biosynthesis, Eukaryotes Pathway (468360); Cytosolic TRNA Aminoacylation Pathway (105981); Gene Expression Pathway (105937); Tryptophan Metabolism Pathway (198850); TRNA Aminoacylation Pathway (105980)

ncbi summary :

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of tryptophanyl-tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. Tryptophanyl-tRNA synthetase (WARS) catalyzes the aminoacylation of tRNA(trp) with tryptophan and is induced by interferon. Tryptophanyl-tRNA synthetase belongs to the class I tRNA synthetase family. Four transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: Isoform 1, isoform 2 and T1-TrpRS have aminoacylation activity while T2-TrpRS lacks it. Isoform 2, T1-TrpRS and T2-TrpRS possess angiostatic activity whereas isoform 1 lacks it. T2-TrpRS inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression.4 PublicationsManual assertion based on experiment in:Ref.14. Catalytic activity: ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage