ELISA/assay

Rabbit receptor activator of nuclear factor kappa B ligand, RANKL ELISA Kit

MBS703289

96 Strip Wells

915 USD

ELISA Kit

Rabbit receptor activator of nuclear factor kappa B ligand, RANKL ELISA Kit

receptor activator of nuclear factor kappa B ligand, RANKL

Rabbit receptor activator of nuclear factor kappa B ligand; RANKL ELISA Kit; receptor activator of nuclear factor kappa B ligand; RANKL

RANKL; Tumor necrosis factor ligand superfamily member 11; tumor necrosis factor ligand superfamily member 11; osteoprotegerin ligand; osteoclast differentiation factor; TNF-related activation-induced cytokine; receptor activator of nuclear factor kappa B ligand; receptor activator of nuclear factor kappa-B ligand; tumor necrosis factor (ligand) superfamily, member 11; Osteoclast differentiation factor; ODF; Osteoprotegerin ligand; OPGL; Receptor activator of nuclear factor kappa-B ligand; RANKL; TNF-related activation-induced cytokine; TRANCE; CD_antigen: CD254Cleaved into the following 2 chains:Tumor necrosis factor ligand superfamily member 11, membrane form; Tumor necrosis factor ligand superfamily member 11, soluble form

RANKL

TNFSF11; TNFSF11; ODF; OPGL; sOdf; CD254; OPTB2; RANKL; TRANCE; hRANKL2; OPGL; RANKL; TRANCE; ODF; OPGL; RANKL; TRANCE

TNF11_HUMAN

Rabbit

317

This assay has high sensitivity and excellent specificity for detection of rabbit RANKL. No significant cross-reactivity or interference between rabbit RANKL and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: For the quantitative determination of rabbit receptor activator of nuclear factor kappa B ligand (RANKL) concentrations in serum, plasma, tissue homogenates. Detection Range: 15.6 pg/ml-1000 pg/ml.

Sensitivity: The minimum detectable dose of rabbit RANKL is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for RANKL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RANKL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RANKL is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RANKL bound in the initial step. The color development is stopped and the intensity of the color is measured.

2612922

AAB86811.1

O14788

30,523 Da

Cytokine-cytokine Receptor Interaction Pathway 83051!!Cytokine-cytokine Receptor Interaction Pathway 460!!IL6-mediated Signaling Events Pathway 137932!!NF-kappa B Signaling Pathway 634527!!Osteoblast Signaling Pathway 198842!!Osteoclast Signaling Pathway 198769!!Osteoclast Differentiation Pathway 193147!!Osteoclast Differentiation Pathway 193096!!Prolactin Signaling Pathway 814182!!Prolactin Signaling Pathway 814248

This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found. [provided by RefSeq, Jul 2008]

Function: Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.1 PublicationManual assertion based on experiment in:Ref.7

96 Strip Wells

915 USD