catalog number :
MBS703285
products type :
ELISA Kit
products full name :
Mouse glutamic acid decarboxylase (GAD) autoantibody, IgM ELISA Kit
products short name :
glutamic acid decarboxylase (GAD) autoantibody (IgM)
products name syn :
Mouse glutamic acid decarboxylase autoantibody IgM; GAD-Ab-IgM ELISA Kit; Mouse glutamic acid decarboxylase autoantibody IgM; GAD-Ab-IgM
other names :
glutamic acid decarboxylase, partial; Glutamate decarboxylase 2; 65 kDa glutamic acid decarboxylase; GAD-65; Glutamate decarboxylase 65 kDa isoform
products gene name :
GAD autoantibody (IgM)
other gene names :
GAD2; GAD65; GAD-65
uniprot entry name :
DCE2_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse GAD autoantibody(IgM). No significant cross-reactivity or interference between mouse GAD autoantibody(IgM) and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: For the qualitative determination of mouse glutamic acid decarboxylase(GAD) autoantibody(IgM) concentrations in serum.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Priciple of the assay: This assay employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with antigen. Samples are pipetted into the wells with anti-mouse IgM conjugated Horseradish Peroxidase (HRP). Any antibodies specific for the antigen present will bind to the pre-coated antigen. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of mouse GAD autoantibody(IgM) bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
AAA52513.1
ncbi mol weight :
65,411 Da
uniprot summary :
Function: Catalyzes the production of GABA. Catalytic activity: L-glutamate = 4-aminobutanoate + CO2. Cofactor: Pyridoxal phosphate.1 PublicationManual assertion based on experiment in:Ref.10