Human Phospholipidase A2, PLA2 ELISA Kit | MyBioSource MBS703283 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Phospholipidase A2, PLA2 ELISA Kit

catalog :

MBS703283

quantity :

96 Strip Wells

price :

480 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS703283

products type :

ELISA Kit

products full name :

Human Phospholipidase A2, PLA2 ELISA Kit

products short name :

phospholipase A2, group IB (pancreas)

products name syn :

Human Phospholipidase A2; PLA2 ELISA Kit; MGC119834; MGC119835; PLA2; PLA2A; PPLA2; phosphatidylcholine 2-acylhydrolase; phospholipase A2 group IB; phospholipase A2; group IB (pancreas)

other names :

phospholipase A2; Phospholipase A2; phospholipase A2; phosphatidylcholine 2-acylhydrolase 1B; phospholipase A2, group IB (pancreas); Group IB phospholipase A2; Phosphatidylcholine 2-acylhydrolase 1B

products gene name :

PLA2G1B

other gene names :

PLA2G1B; PLA2G1B; PLA2; PLA2A; PPLA2; PLA2; PLA2A; PPLA2

uniprot entry name :

PA21B_HUMAN

reactivity :

Human

sequence length :

148

specificity :

This assay has high sensitivity and excellent specificity for detection of Human PLA2G1B. No significant cross-reactivity or interference between Human PLA2G1B and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma and tissue homogenates. Detection Range: 0.45 ng/ml-30 ng/ml. Detection Wavelength: 450 nm. Sensitivity: 0.11 ng/ml

other info2 :

Sample Volume: 50-100ul. Protein Biological Process 1: Biosynthesis/Metabolism. Protein Biological Process 2: Lipogenesis and lipometabolism. Protein Biological Process 3: Lipid degradation

products description :

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PLA2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for PLA2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain PLA2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of PLA2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

4505847

ncbi acc num :

NP_000919.1

ncbi gb acc num :

NM_000928.2

uniprot acc num :

P04054

ncbi mol weight :

16,360 Da

ncbi pathways :

Acyl Chain Remodelling Of PC Pathway 645321!!Acyl Chain Remodelling Of PE Pathway 645317!!Acyl Chain Remodelling Of PG Pathway 645328!!Acyl Chain Remodelling Of PI Pathway 645326!!Acyl Chain Remodelling Of PS Pathway 645324!!Arachidonic Acid Metabolism Pathway 82991!!Arachidonic Acid Metabolism Pathway 366!!Ether Lipid Metabolism Pathway 82990!!Ether Lipid Metabolism Pathway 365!!Fat Digestion And Absorption Pathway 194385

ncbi summary :

This gene encodes a secreted member of the phospholipase A2 (PLA2) class of enzymes, which is produced by the pancreatic acinar cells. The encoded calcium-dependent enzyme catalyzes the hydrolysis of the sn-2 position of membrane glycerophospholipids to release arachidonic acid (AA) and lysophospholipids. AA is subsequently converted by downstream metabolic enzymes to several bioactive lipophilic compounds (eicosanoids), including prostaglandins (PGs) and leukotrienes (LTs). The enzyme may be involved in several physiological processes including cell contraction, cell proliferation and pathological response. [provided by RefSeq, Aug 2013]

uniprot summary :

Function: PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides, this releases glycerophospholipids and arachidonic acid that serve as the precursors of signal molecules.1 PublicationManual assertion based on experiment in:Ref.9. Catalytic activity: Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate. Cofactor: Binds 1 calcium ion per subunit

size :

96 Strip Wells

price :

480 USD

more info or order :

MyBioSource product webpage