catalog number :
MBS703282
products type :
ELISA Kit
products full name :
Rat placenta growth factor, PLGF ELISA Kit
products short name :
placental growth factor
products name syn :
Rat placenta growth factor; PLGF ELISA kit; D12S1900; PGFL; PLGF; PlGF-2; SHGC-10760; placenta growth factor; placental growth factor; vascular endothelial growth factor-related protein; placental growth factor-like; placental growth factor
other names :
placenta growth factor; Placenta growth factor; placenta growth factor; placental growth factor
other gene names :
Pgf; Pgf; Plgf; Plgf; PlGF
uniprot entry name :
PLGF_RAT
specificity :
This assay has high sensitivity and excellent specificity for detection of rat PLGF. No significant cross-reactivity or interference between rat PLGF and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 3.12 pg/ml -200 pg/ml. Sensitivity: 0.78 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PLGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PLGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLGF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLGF bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_446047.1
ncbi gb acc num :
NM_053595.2
ncbi mol weight :
17,681 Da
ncbi pathways :
Cardiovascular Signaling Pathway (219769); Focal Adhesion Pathway (83459); Focal Adhesion Pathway (478); PI3K-Akt Signaling Pathway (692247); PI3K-Akt Signaling Pathway (692979); Pathways In Cancer (83493); Rap1 Signaling Pathway (869320); Rap1 Signaling Pathway (878042); Ras Signaling Pathway (869318); Signal Transduction Pathway (1010015)
ncbi summary :
forms a heterodimer with VEGF; may play a role in vascular endothelial cell proliferation [RGD, Feb 2006]
uniprot summary :
Function: Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Also promotes cell tumor growth
size4 :
10x96-Strip-Wells