ELISA/assay

Rat cyclooxygenase-2, COX-2 ELISA Kit

MBS703280

48-Strip-Wells

510 USD

ELISA Kit

Rat cyclooxygenase-2, COX-2 ELISA Kit

prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)

Rat cyclooxygenase-2; COX-2 ELISA Kit; COX-2; COX2; GRIPGHS; PGG/HS; PGHS-2; PHS-2; hCox-2; cyclooxygenase 2b; prostaglandin G/H synthase and cyclooxygenase; prostaglandin-endoperoxide synthase 2; prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)

prostaglandin G/H synthase 2; Prostaglandin G/H synthase 2; prostaglandin G/H synthase 2; PGHS-2; PHS II; PGH synthase 2; cyclooxygenase-2; prostaglandin H2 synthase 2; Cyclooxygenase-2; COX-2; PHS II; Prostaglandin H2 synthase 2; PGH synthase 2; PGHS-2; Prostaglandin-endoperoxide synthase 2

PTGS2

PTGS2; PTGS2; COX-2; COX-2; COX2; COX-2; PGH synthase 2; PGHS-2

PGH2_RABIT

Rat

604

This assay has high sensitivity and excellent specificity for detection of rat COX-2. No significant cross-reactivity or interference between rat COX-2 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 1.56 ng/ml -100 ng/ml. Sensitivity: The minimum detectable dose of rat COX-2 is typically less than 0.39 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for COX-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any COX-2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for COX-2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of COX-2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

126723503

NP_001075857.1

NM_001082388.1

O02768

69,007 Da

Arachidonic Acid Metabolism Pathway (82991); Arachidonic Acid Metabolism Pathway (366); Biological Oxidations Pathway (105698); C-MYB Transcription Factor Network Pathway (138073); COX Reactions Pathway (105711); Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway (137993); Calcium Signaling In The CD4+ TCR Pathway (137941); Eicosanoid Synthesis Pathway (198888); Leishmaniasis Pathway (144181); Leishmaniasis Pathway (144028)

Function: Converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis. Involved in the constitutive production of prostanoids in particular in the stomach and platelets. In gastric epithelial cells, it is a key step in the generation of prostaglandins, such as prostaglandin E2 (PGE2), which plays an important role in cytoprotection. In platelets, it is involved in the generation of thromboxane A2 (TXA2), which promotes platelet activation and aggregation, vasoconstriction and proliferation of vascular smooth muscle cells . Catalytic activity: Arachidonate + AH2 + 2 O2 = prostaglandin H2 + A + H2O. Cofactor: Binds 1 heme B (iron-protoporphyrin IX) group per subunit

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800