ELISA/assay

Human type II collagen helical peptide, HELIX-II ELISA Kit

MBS703265

96 Strip Wells

850 USD

ELISA Kit

Human type II collagen helical peptide, HELIX-II ELISA Kit

type II collagen helical peptide, HELIX-II

Human type II collagen helical peptide; HELIX-IIELISA Kit; type II collagen helical peptide; HELIX-II

HELIXII

Human

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Sample Type: serum, plasma, urine. Detection Range: 1.25 ng/ml-80 ng/ml. Detection Wavelength: 450 nm. Sensitivity: 0.312 ng/ml

Sample Volume: 50-100ul

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to HELIX-II. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for HELIX-II and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain HELIX-II, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of HELIX-II in the samples is then determined by comparing the O.D. of the samples to the standard curve.

96 Strip Wells

850 USD