Human Neuroglobin, NGB ELISA Kit | MyBioSource MBS703263 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Neuroglobin, NGB ELISA Kit

catalog :

MBS703263

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS703263

products type :

ELISA Kit

products full name :

Human Neuroglobin, NGB ELISA Kit

products short name :

neuroglobin

products name syn :

Human Neuroglobin; NGB ELISA Kit; neuroglobin

other names :

neuroglobin; Neuroglobin; neuroglobin; neuroglobin

products gene name :

NGB

other gene names :

NGB; NGB

uniprot entry name :

NGB_HUMAN

reactivity :

Human

sequence length :

151

specificity :

This assay has high sensitivity and excellent specificity for detection of Human NGB. No significant cross-reactivity or interference between Human NGB and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, urine. Detection Range: 1.87 ng/ml-120 ng/ml. Sensitivity: 0.46 ng/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Transport. Protein Biological Process 2: Anion transport. Protein Biological Process 3: Oxygen transport

products description :

Introduction: Neuroglobin is a member of the vertebrate globin family involved in cellular oxygen homeostasis. It is an intracellular hemoprotein expressed in the central and peripheral nervous system, cerebrospinal fluid, retina and endocrine tissues. Neuroglobin is a monomer that reversibly binds oxygen with an affinity higher than that of hemoglobin. It also increases oxygen availability to brain tissue and provides protection under hypoxic or ischemic conditions, potentially limiting brain damage. It is of ancient evolutionary origin, and is homologous to nerve globins of invertebrates. Neuroglobin was first identified by Thorsten Burmester et al. in 2000. Italian researchers suggest that neuroglobin is more likely to usher in nitric oxide to protect neuron survival and recovery in areas where oxygen supply is reduced. The 3D structure of human neuroglobin was determined in 2003. The next year, murine neuroglobin was determined at a higher resolution. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NGB. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for NGB and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain NGB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of NGB in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

10864065

ncbi acc num :

NP_067080.1

ncbi gb acc num :

NM_021257.3

uniprot acc num :

Q9NPG2

ncbi mol weight :

16,933 Da

ncbi pathways :

Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)

ncbi summary :

This gene encodes an oxygen-binding protein that is distantly related to members of the globin gene family. It is highly conserved among other vertebrates. It is expressed in the central and peripheral nervous system where it may be involved in increasing oxygen availability and providing protection under hypoxic/ischemic conditions. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: Involved in oxygen transport in the brain. Hexacoordinate globin, displaying competitive binding of oxygen or the distal His residue to the iron atom. Not capable of penetrating cell membranes. The deoxygenated form exhibits nitrite reductase activity inhibiting cellular respiration via NO-binding to cytochrome c oxidase. Involved in neuroprotection during oxidative stress. May exert its anti-apoptotic activity by acting to reset the trigger level of mitochondrial cytochrome c release necessary to commit the cells to apoptosis.5 PublicationsManual assertion based on experiment in:Ref.1

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage