ELISA/assay

Human Hyaluronidase-1, HYAL1 ELISA Kit

MBS703230

48-Strip-Wells

510 USD

ELISA Kit

Human Hyaluronidase-1, HYAL1 ELISA Kit

hyaluronoglucosaminidase 1

Human Hyaluronidase-1 (HYAL1) ELISA kit; HYAL-1; LUCA1; MGC45987; NAT6; OTTHUMP00000211076; hyaluronidase 1; plasma hyaluronidase; tumor suppressor LUCA-1; hyaluronoglucosaminidase 1

hyaluronidase-1 isoform 1; Hyaluronidase-1; hyaluronidase-1; luCa-1; plasma hyaluronidase; tumor suppressor LUCA-1; lung carcinoma protein 1; hyaluronoglucosaminidase 1; Hyaluronoglucosaminidase-1; Lung carcinoma protein 1; LuCa-1

HYAL1

HYAL1; HYAL1; NAT6; LUCA1; HYAL-1; LUCA1; Hyal-1; LuCa-1

HYAL1_HUMAN

Human

435

This assay has high sensitivity and excellent specificity for detection of human HYAL1. No significant cross-reactivity or interference between human HYAL1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 1.25 ng/ml -80 ng/ml. Sensitivity: 0.31 ng/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HYAL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HYAL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HYAL1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HYAL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

24497562

NP_149349.2

NM_033159.3

Q12794

48,368 Da

CS/DS Degradation Pathway (645311); Chondroitin Sulfate Degradation Pathway (413376); Chondroitin Sulfate Degradation Pathway (468247); Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway (645308); Dermatan Sulfate Degradation Pathway (413375); Dermatan Sulfate Degradation Pathway (468240); Disease Pathway (530764); Glycogen Storage Diseases Pathway (980468); Glycosaminoglycan Degradation Pathway (82981); Glycosaminoglycan Degradation Pathway (355)

This gene encodes a lysosomal hyaluronidase. Hyaluronidases intracellularly degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan is thought to be involved in cell proliferation, migration and differentiation. This enzyme is active at an acidic pH and is the major hyaluronidase in plasma. Mutations in this gene are associated with mucopolysaccharidosis type IX, or hyaluronidase deficiency. The gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Function: May have a role in promoting tumor progression. May block the TGFB1-enhanced cell growth.1 PublicationManual assertion based on experiment in:Ref.4. Catalytic activity: Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800