catalog number :
MBS703226
products type :
ELISA Kit
products full name :
Human Interleukin 25, IL-25 ELISA Kit
products short name :
interleukin 25
products name syn :
Human Interleukin 25; IL-25 ELISA kit; IL-17E; IL-25; IL17E; interleukin 17E; interleukin 25
other names :
interleukin-25 isoform 1; Interleukin-25; interleukin-25; interleukin-17E; interleukin 25; Interleukin-17E; IL-17E
products gene name :
IL25
other gene names :
IL25; IL25; IL17E; IL17E; UNQ3120/PRO10272; IL-25; IL-17E
uniprot entry name :
IL25_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of human IL-25. No significant cross-reactivity or interference between human IL-25 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, cell culture supernates, urine, tissue homogenates. Assay Type: Sandwich. Detection Range: 62.5 pg/ml -4000 pg/ml. Sensitivity: 15.6 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL-25 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-25 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-25 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-25 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_073626.1
ncbi gb acc num :
NM_022789.3
ncbi mol weight :
20,330 Da
ncbi pathways :
Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); IL17 Signaling Pathway (920968)
ncbi summary :
The protein encoded by this gene is a cytokine that shares sequence similarity with interleukin 17. This cytokine can induce NF-kappaB activation, and stimulate the production of interleukin 8. Both this cytokine and interleukin 17B are ligands for the cytokine receptor IL17BR. Studies of a similar gene in mice suggest that this cytokine may be a pro-inflammatory cytokine favoring the Th2-type immune response. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]
uniprot summary :
Function: Induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. Proinflammatory cytokine favoring Th2-type immune responses.
size4 :
10x96-Strip-Wells