Human Neural -Cadherin, N-Cad ELISA Kit | MyBioSource MBS703223 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Neural -Cadherin, N-Cad ELISA Kit

catalog :

MBS703223

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS703223

products type :

ELISA Kit

products full name :

Human Neural -Cadherin, N-Cad ELISA Kit

products short name :

cadherin 2, type 1, N-cadherin (neuronal)

products name syn :

Human Neural -Cadherin; N-Cad ELISA kit; CD325; CDHN; CDw325; NCAD; N-cadherin 1; cadherin 2; N-cadherin (neuronal) ; cadherin 2; type 1; calcium-dependent adhesion protein; neuronal; neural cadherin; cadherin 2; type 1; N-cadherin (neuronal)

other names :

cadherin-2 preproprotein; Cadherin-2; cadherin-2; N-cadherin 1; neural cadherin; neural-cadherin; cadherin 2, N-cadherin (neuronal); calcium-dependent adhesion protein, neuronal; cadherin 2, type 1, N-cadherin (neuronal); CDw325; Neural cadherin; N-cadherin; CD_antigen: CD325

products gene name :

CDH2

other gene names :

CDH2; CDH2; CDHN; NCAD; CD325; CDw325; CDHN; NCAD; N-cadherin

uniprot entry name :

CADH2_HUMAN

reactivity :

Human

sequence length :

906

specificity :

This assay has high sensitivity and excellent specificity for detection of human N-Cad. No significant cross-reactivity or interference between human N-Cad and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 6.25 ng/ml -400 ng/ml. Sensitivity: The minimum detectable dose of human N-Cad is typically less than 1.56 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for N-Cad has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any N-Cad present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for N-Cad is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of N-Cad bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

14589889

ncbi acc num :

NP_001783.2

ncbi gb acc num :

NM_001792.3

uniprot acc num :

P19022

ncbi mol weight :

99,809 Da

ncbi pathways :

Adherens Junctions Interactions Pathway (119533); Arrhythmogenic Right Ventricular Cardiomyopathy Pathway (672454); Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (117293); Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (116129); BDNF Signaling Pathway (712093); CDO In Myogenesis Pathway (160998); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Cell Junction Organization Pathway (160966); Cell-Cell Communication Pathway (477132)

ncbi summary :

This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage