catalog number :
MBS703221
products type :
ELISA Kit
products full name :
Mouse Haptoglobin, Hpt/HP ELISA Kit
products short name :
haptoglobin
products name syn :
Mouse Haptoglobin; Hpt/HP ELISA KIT; BP; HP2-ALPHA-2; HPA1S; MGC111141; binding peptide; haptoglobin alpha (1S) -beta; haptoglobin alpha (2FS) -beta; haptoglobin; alpha polypeptide; haptoglobin; beta polypeptide; haptoglobin
other names :
haptoglobin; Haptoglobin; haptoglobin; zonulin; haptoglobin; ZonulinCleaved into the following 2 chains:Haptoglobin alpha chain; Haptoglobin beta chain
other gene names :
Hp; Hp; HP-1; preHP2
uniprot entry name :
HPT_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of Mouse HP. No significant cross-reactivity or interference between Mouse HP and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma. Detection Range: 0.156 ug/ml-10 ug/ml. Sensitivity: 0.039 ug/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul
products description :
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with Hpt. Standards or samples are then added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) -conjugated antibody preparation specific for Hpt, mix well and incubated. The more the amount of Hpt in samples, the less HRP- conjugated antibody preparation specific for Hpt bound by pre-coated Hpt. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. And the color develops in opposite to the amount of Hpt in the sample. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_059066.1
ncbi gb acc num :
NM_017370.2
ncbi mol weight :
38,752 Da
ncbi pathways :
Binding And Uptake Of Ligands By Scavenger Receptors Pathway (1000591); Scavenging Of Heme From Plasma Pathway (1000592)
uniprot summary :
Function: As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway
size4 :
10x96-Strip-Wells