Human Sialidase ELISA Kit | MyBioSource MBS703211 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Sialidase ELISA Kit

catalog :

MBS703211

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS703211

products type :

ELISA Kit

products full name :

Human Sialidase ELISA Kit

products short name :

sialidase 1 (lysosomal sialidase)

products name syn :

Human Sialidase ELISA Kit; DAAP-222I20.1; FLJ93471; NANH; NEU; SIAL1; G9 sialidase; N-acetyl-alpha-neuraminidase 1; acetylneuraminyl hydrolase; exo-alpha-sialidase; lysosomal sialidase; neuraminidase 1; sialidase 1 (lysosomal sialidase)

other names :

sialidase-1; Sialidase-1; sialidase-1; G9 sialidase; exo-alpha-sialidase; lysosomal sialidase; acetylneuraminyl hydrolase; N-acetyl-alpha-neuraminidase 1; sialidase 1 (lysosomal sialidase); Acetylneuraminyl hydrolase; G9 sialidase; Lysosomal sialidase; N-acetyl-alpha-neuraminidase 1

products gene name :

NEU1

other gene names :

NEU1; NEU1; NEU; NANH; SIAL1; NANH

uniprot entry name :

NEUR1_HUMAN

reactivity :

Human

sequence length :

415

specificity :

This assay has high sensitivity and excellent specificity for detection of human sialidase. No significant cross-reactivity or interference between human sialidase and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, urine, cerebrospinal fluid (CSF). Assay Type: Quantitative Sandwich. Sensitivity: Typically less than 0.078 ng/ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for sialidase has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any sialidase present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for sialidase is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of sialidase bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

4557791

ncbi acc num :

NP_000425.1

ncbi gb acc num :

NM_000434.3

uniprot acc num :

Q99519

ncbi mol weight :

45,467 Da

ncbi pathways :

Asparagine N-linked Glycosylation Pathway (161013); Biosynthesis Of The N-glycan Precursor (dolichol Lipid-linked Oligosaccharide, LLO) And Transfer To A Nascent Protein Pathway (161014); Glycosphingolipid Metabolism Pathway (530751); Lysosome Pathway (99052); Lysosome Pathway (96865); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Metabolism Of Proteins Pathway (106230); Other Glycan Degradation Pathway (82976); Other Glycan Degradation Pathway (346)

ncbi summary :

The protein encoded by this gene is a lysosomal enzyme that cleaves terminal sialic acid residues from substrates such as glycoproteins and glycolipids. In the lysosome, this enzyme is part of a heterotrimeric complex together with beta-galactosidase and cathepsin A (the latter is also referred to as 'protective protein'). Mutations in this gene can lead to sialidosis, a lysosomal storage disease that can be type 1 (cherry red spot-myoclonus syndrome or normosomatic type), which is late-onset, or type 2 (the dysmorphic type), which occurs at an earlier age with increased severity. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: Catalyzes the removal of sialic acid (N-acetylneuraminic acid) moities from glycoproteins and glycolipids. To be active, it is strictly dependent on its presence in the multienzyme complex. Appears to have a preference for alpha 2-3 and alpha 2-6 sialyl linkage.1 PublicationManual assertion based on experiment in:Ref.1. Catalytic activity: Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.3 PublicationsManual assertion based on experiment in:Ref.1

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage