Human collagen, type I, alpha 1, COL1A1 ELISA Kit | MyBioSource MBS703198 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human collagen, type I, alpha 1, COL1A1 ELISA Kit

catalog :

MBS703198

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS703198

products type :

ELISA Kit

products full name :

Human collagen, type I, alpha 1, COL1A1 ELISA Kit

products short name :

collagen, type I, alpha 1

products name syn :

Human collagen; type I; alpha 1 (COL1A1) ELISA kit; OI4; alpha 1 type I collagen; collagen alpha 1 chain type I; collagen of skin; tendon and bone; alpha-1 chain; pro-alpha-1 collagen type 1; collagen; type I; alpha 1

other names :

collagen alpha-1(I) chain preproprotein; Collagen alpha-1(I) chain; collagen alpha-1(I) chain; collagen alpha-1(I) chain; alpha-1 type I collagen; pro-alpha-1 collagen type 1; collagen alpha 1 chain type I; collagen alpha-1(I) chain preproprotein; collagen of skin, tendon and bone, alpha-1 chain; collagen, type I, alpha 1; Alpha-1 type I collagen

products gene name :

COL1A1

other gene names :

COL1A1; COL1A1; OI4

uniprot entry name :

CO1A1_HUMAN

reactivity :

Human

sequence length :

1464

specificity :

This assay has high sensitivity and excellent specificity for detection of human COL1A1. No significant cross-reactivity or interference between human COL1A1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.625 ng/ml -40 ng/ml. Sensitivity: 0.156 ng/ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for COL1A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any COL1A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for COL1A1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of COL1A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

110349772

ncbi acc num :

NP_000079.2

ncbi gb acc num :

NM_000088.3

uniprot acc num :

P02452

ncbi mol weight :

138,941 Da

ncbi pathways :

Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (730306); Binding And Uptake Of Ligands By Scavenger Receptors Pathway (771599); Collagen Biosynthesis And Modifying Enzymes Pathway (645289); Collagen Formation Pathway (645288); ECM-receptor Interaction Pathway (83068); ECM-receptor Interaction Pathway (479); Extracellular Matrix Organization Pathway (576262); Focal Adhesion Pathway (198795)

ncbi summary :

This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish, Feb 2008]

uniprot summary :

Function: Type I collagen is a member of group I collagen (fibrillar forming collagen).

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage