ELISA/assay

Human Complement Fragment 4d, C4d ELISA Kit

MBS703196

96 Strip Wells

760 USD

ELISA Kit

Human Complement Fragment 4d, C4d ELISA Kit

Complement Fragment 4d, C4d

Human Complement Fragment 4d; C4d ELISA Kit; Complement Fragment 4d; C4d

C4d

Human

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Sample Type: serum, plasma, tissue homogenates. Detection Range: 0.78 ng/ml-50 ng/ml. Detection Wavelength: 450 nm. Sensitivity: 0.195 ng/ml

Sample Volume: 50-100ul

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to C4d. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for C4d and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain C4d, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of C4d in the samples is then determined by comparing the O.D. of the samples to the standard curve.

96 Strip Wells

760 USD