ELISA/assay

Rat inducible nitric oxide synthase, iNOS ELISA Kit

MBS703177

48-Strip-Wells

565 USD

ELISA Kit

Rat inducible nitric oxide synthase, iNOS ELISA Kit

nitric oxide synthase 2, inducible

Rat inducible nitric oxide synthase; iNOS ELISA KIT; HEP-NOS; INOS; NOS; NOS2A; NOS; type II; nitric oxide synthase 2A; nitric oxide synthase 2A (inducible; hepatocytes) ; nitric oxide synthase; macrophage; nitric oxide synthase 2; inducible

Nitric oxide synthase, inducible; Nitric oxide synthase, inducible; Inducible NO synthase; Inducible NOS; iNOS; NOS type II; Peptidyl-cysteine S-nitrosylase NOS2

NOS2

NOS2; Inducible NOS; iNOS

NOS2_RABIT

Rat

496

This assay has high sensitivity and excellent specificity for detection of rat iNOS. No significant cross-reactivity or interference between rat iNOS and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.78 IU/ml -50 IU/ml. Sensitivity: The minimum detectable dose of rat iNOS is typically less than 0.195 IU/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

. Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for iNOS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any iNOS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for iNOS is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of iNOS bound in the initial step. The color development is stopped and the intensity of the color is measured.

8473491

O19114.1

O19114

56,397 Da

Inositol Phosphate Metabolism Pathway (3024); Inositol Phosphate Metabolism Pathway (362)

Function: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2 . Catalytic activity: 2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O. Cofactor: Heme group . Enzyme regulation: Not stimulated by calcium/calmodulin

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150