catalog number :
MBS703158
products type :
ELISA Kit
products full name :
Human Hyaluronic acid, HA ELISA Kit
products short name :
[Hyaluronic acid, HA]
products name syn :
[Human Hyaluronic acid; HA ELISA Kit; Hyaluronic acid; HA]
other names :
[hyaluronic acid receptor; Lymphatic vessel endothelial hyaluronic acid receptor 1; lymphatic vessel endothelial hyaluronic acid receptor 1; extracellular link domain containing 1; extracellular link domain-containing 1; extracellular link domain-containing protein 1; cell surface retention sequence binding protein-1; cell surface retention sequence-binding protein 1; lymphatic vessel endothelial hyaluronan receptor 1; Cell surface retention sequence-binding protein 1; CRSBP-1; Extracellular link domain-containing protein 1; Hyaluronic acid receptor]
products gene name :
[HA]
other gene names :
[LYVE1; LYVE1; HAR; XLKD1; LYVE-1; CRSBP-1; CRSBP1; HAR; XLKD1; UNQ230/PRO263; LYVE-1; CRSBP-1]
uniprot entry name :
LYVE1_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse GZMA. No significant cross-reactivity or interference between mouse GZMA and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Tissue Homogenates. Assay Type: Quantitative Sandwich. Detection Range: 6.25 pg/ml-400 pg/ml. Sensitivity: 1.56 pg/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GZMA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GZMA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GZMA is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GZMA bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
AAD49220.2
ncbi mol weight :
35,213 Da
ncbi pathways :
Disease Pathway (530764); Glycogen Storage Diseases Pathway (980468); Glycosaminoglycan Metabolism Pathway (645297); Hyaluronan Metabolism Pathway (645298); Hyaluronan Uptake And Degradation Pathway (645300); MPS I - Hurler Syndrome Pathway (685537); MPS II - Hunter Syndrome Pathway (685538); MPS IIIA - Sanfilippo Syndrome A Pathway (685539); MPS IIIB - Sanfilippo Syndrome B Pathway (685540); MPS IIIC - Sanfilippo Syndrome C Pathway (685541)
ncbi summary :
This gene encodes a type I integral membrane glycoprotein. The encoded protein acts as a receptor and binds to both soluble and immobilized hyaluronan. This protein may function in lymphatic hyaluronan transport and have a role in tumor metastasis. [provided by RefSeq, Jul 2008]
uniprot summary :
Function: Ligand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. Plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). May act as a hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes.1 PublicationManual assertion based on experiment in:Ref.1
size1 :
24-Strip-Wells (LIMIT 1)
size5 :
10x96-Strip-Wells