Human Lecithin Cholesterol Acyltransferase, LCat ELISA Kit | MyBioSource MBS703156 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Lecithin Cholesterol Acyltransferase, LCat ELISA Kit

catalog :

MBS703156

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS703156

products type :

ELISA Kit

products full name :

Human Lecithin Cholesterol Acyltransferase, LCat ELISA Kit

products short name :

lecithin-cholesterol acyltransferase

products name syn :

Human Lecithin Cholesterol Acyltransferase (LCAT) ELISA Kit; lecithin-cholesterol acyltransferase

other names :

phosphatidylcholine-sterol acyltransferase; Phosphatidylcholine-sterol acyltransferase; phosphatidylcholine-sterol acyltransferase; phospholipid-cholesterol acyltransferase; lecithin-cholesterol acyltransferase; Lecithin-cholesterol acyltransferase; Phospholipid-cholesterol acyltransferase

products gene name :

LCAT

other gene names :

LCAT; LCAT

reactivity :

Human

sequence length :

440

specificity :

This assay has high sensitivity and excellent specificity for detection of human LCAT. No significant cross-reactivity or interference between human LCAT and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma and tissue homogenates. Assay Type: Sandwich. Detection Range: 3.12 ng/ml -200 ng/ml. Sensitivity: 0.78 ng/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LCAT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LCAT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LCAT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LCAT bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

4557892

ncbi acc num :

NP_000220.1

ncbi gb acc num :

NM_000229.1

uniprot acc num :

P04180

ncbi mol weight :

49,578 Da

ncbi pathways :

Glycerophospholipid Metabolism Pathway (82989); Glycerophospholipid Metabolism Pathway (364); HDL-mediated Lipid Transport Pathway (106158); Lipid Digestion, Mobilization, And Transport Pathway (106111); Lipoprotein Metabolism Pathway (106156); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Statin Pathway (198852)

ncbi summary :

This gene encodes the extracellular cholesterol esterifying enzyme, lecithin-cholesterol acyltransferase. The esterification of cholesterol is required for cholesterol transport. Mutations in this gene have been found to cause fish-eye disease as well as LCAT deficiency. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: Central enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs). The cholesterol ester is then transported back to the liver. Has a preference for plasma 16:0-18:2 or 18:O-18:2 phosphatidylcholines. Also produced in the brain by primary astrocytes, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels. Together with APOE and the cholesterol transporter ABCA1, plays a key role in the maturation of glial-derived, nascent lipoproteins. Required for remodeling high-density lipoprotein particles into their spherical forms.1 PublicationManual assertion based on experiment in:Ref.15. Catalytic activity: Phosphatidylcholine + a sterol = 1-acylglycerophosphocholine + a sterol ester. Enzyme regulation: APOA1 is the most potent activator in plasma. Also activated by APOE, APOC1 and APOA4.1 PublicationManual assertion based on experiment in:Ref.19

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage