ELISA/assay

Human chaperonin 10, CPN10 ELISA Kit

MBS703143

48-Strip-Wells

565 USD

ELISA Kit

Human chaperonin 10, CPN10 ELISA Kit

heat shock 10kDa protein 1 (chaperonin 10)

Human chaperonin 10; CPN10 ELISA Kit; CPN10; GROES; HSP10; chaperonin 10; heat shock 10kD protein 1 (chaperonin 10) ; heat shock 10kDa protein 1; heat shock 10kDa protein 1 (chaperonin 10)

10 kDa heat shock protein, mitochondrial; 10 kDa heat shock protein, mitochondrial; 10 kDa heat shock protein, mitochondrial; chaperonin 10; 10 kDa chaperonin; early-pregnancy factor; heat shock 10kD protein 1 (chaperonin 10); heat shock 10kDa protein 1; 10 kDa chaperonin; Chaperonin 10; CPN10; Early-pregnancy factor; EPF

HSPE1

HSPE1; HSPE1; EPF; CPN10; GROES; HSP10; Hsp10; CPN10; EPF

CH10_HUMAN

Human

102

This assay has high sensitivity and excellent specificity for detection of human CPN10. No significant cross-reactivity or interference between human CPN10 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.16 ng/ml -10 ng/ml. Sensitivity: 0.04 ng/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CPN10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CPN10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CPN10 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CPN10 bound in the initial step. The color development is stopped and the intensity of the color is measured.

4504523

NP_002148.1

NM_002157.2

P61604

10,932 Da

Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)

This gene encodes a major heat shock protein which functions as a chaperonin. Its structure consists of a heptameric ring which binds to another heat shock protein in order to form a symmetric, functional heterodimer which enhances protein folding in an ATP-dependent manner. This gene and its co-chaperonin, HSPD1, are arranged in a head-to-head orientation on chromosome 2. Naturally occurring read-through transcription occurs between this locus and the neighboring locus MOBKL3.[provided by RefSeq, Feb 2011]

Function: Eukaryotic CPN10 homolog which is essential for mitochondrial protein biogenesis, together with CPN60. Binds to CPN60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter.UniRule annotationHAMAP-Rule:MF_00580

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150