Human Thrombin activatable fibrinolysis inhibitor, TAFI ELISA Kit | MyBioSource MBS703133 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Thrombin activatable fibrinolysis inhibitor, TAFI ELISA Kit

catalog :

MBS703133

quantity :

96-Strip-Wells

price :

1025 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS703133

products type :

ELISA Kit

products full name :

Human Thrombin activatable fibrinolysis inhibitor, TAFI ELISA Kit

products short name :

carboxypeptidase B2 (plasma)

products name syn :

Human Thrombin activatable fibrinolysis inhibitor; TAFI ELISA Kit; CPU; PCPB; TAFI; carboxypeptidase B-like protein; carboxypeptidase B2 (plasma; carboxypeptidase U) ; carboxypeptidase U; plasma carboxypeptidase B2; thrombin-activable fibrinolysis inhibitor; thrombin-act; carboxypeptidase B2 (plasma)

other names :

carboxypeptidase B2 isoform 2 preproprotein; Carboxypeptidase B2; carboxypeptidase B2; carboxypeptidase R; carboxypeptidase B-like protein; thrombin-activable fibrinolysis inhibitor; thrombin-activatable fibrinolysis inhibitor; carboxypeptidase B2 (plasma, carboxypeptidase U); carboxypeptidase B2 (plasma); Carboxypeptidase U; CPU; Plasma carboxypeptidase B; pCPB; Thrombin-activable fibrinolysis inhibitor; TAFI

products gene name :

CPB2

other gene names :

CPB2; CPB2; CPU; PCPB; TAFI; CPU; pCPB; TAFI

uniprot entry name :

CBPB2_HUMAN

reactivity :

Human

sequence length :

386

specificity :

This assay has high sensitivity and excellent specificity for detection of human TAFI. No significant cross-reactivity or interference between human TAFI and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma and tissue homogenates. Assay Type: Competitive. Detection Range: 3.12 ng/ml -200 ng/ml. Sensitivity: The minimum detectable dose of human TAFI is typically less than 0.78 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with TAFI. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for TAFI. The competitive inhibition reaction is launched between with pre-coated TAFI and TAFI in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of TAFI in the samples. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

513126877

ncbi acc num :

NP_001265470.1

ncbi gb acc num :

NM_001278541.1

uniprot acc num :

Q96IY4

ncbi mol weight :

48,424 Da

ncbi pathways :

Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Metabolism Of Angiotensinogen To Angiotensins Pathway (685555); Metabolism Of Proteins Pathway (106230); Pancreatic Secretion Pathway (169306); Pancreatic Secretion Pathway (169295); Peptide Hormone Metabolism Pathway (771603); Protein Digestion And Absorption Pathway (172847); Protein Digestion And Absorption Pathway (171868)

ncbi summary :

Carboxypeptidases are enzymes that hydrolyze C-terminal peptide bonds. The carboxypeptidase family includes metallo-, serine, and cysteine carboxypeptidases. According to their substrate specificity, these enzymes are referred to as carboxypeptidase A (cleaving aliphatic residues) or carboxypeptidase B (cleaving basic amino residues). The protein encoded by this gene is activated by trypsin and acts on carboxypeptidase B substrates. After thrombin activation, the mature protein downregulates fibrinolysis. Polymorphisms have been described for this gene and its promoter region. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]

uniprot summary :

Function: Cleaves C-terminal arginine or lysine residues from biologically active peptides such as kinins or anaphylatoxins in the circulation thereby regulating their activities. Down-regulates fibrinolysis by removing C-terminal lysine residues from fibrin that has already been partially degraded by plasmin.1 PublicationManual assertion based on experiment in:Ref.8. Catalytic activity: Release of C-terminal Arg and Lys from a polypeptide.1 PublicationManual assertion based on experiment in:Ref.8. Cofactor: Binds 1 zinc ion per subunit. Enzyme regulation: TAFI/CPB2 is unique among carboxypeptidases in that it spontaneously inactivates with a short half-life, a property that is crucial for its role in controlling blood clot lysis. The zymogen is stabilized by interactions with the activation peptide. Release of the activation peptide increases a dynamic flap mobility and in time this leads to conformational changes that disrupt the catalytic site and expose a cryptic thrombin-cleavage site present at Arg-324.1 PublicationManual assertion based on experiment in:Ref.13

size1 :

96-Strip-Wells

price1 :

1025 USD

size2 :

5x96-Strip-Wells

price2 :

2750

size3 :

10x96-Strip-Wells

price3 :

5150

more info or order :

MyBioSource product webpage