catalog number :
MBS703119
products type :
ELISA Kit
products full name :
Human Transforming Growth factor beta3, TGF-beta3 ELISA Kit
products short name :
transforming growth factor, beta 3
products name syn :
Human Transforming Growth factor beta3; TGF-beta3 ELISA kit; ARVD; FLJ16571; TGF-beta3; ; transforming growth factor; beta 3
other names :
transforming growth factor beta-3 preproprotein; Transforming growth factor beta-3; transforming growth factor beta-3; TGF-beta-3; prepro-transforming growth factor beta-3; transforming growth factor, beta 3
products gene name :
TGFB3
other gene names :
TGFB3; TGFB3; ARVD; RNHF; ARVD1; TGF-beta3; TGF-beta-3; LAP
uniprot entry name :
TGFB3_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of human TGF-beta3. No significant cross-reactivity or interference between human TGF-beta3 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma and cell culture supernates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml -1000 pg/ml. Sensitivity: The minimum detectable dose of human TGF-beta3 is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TGF-beta3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TGF-beta3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TGF-beta3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TGF-beta3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_003230.1
ncbi gb acc num :
NM_003239.3
ncbi mol weight :
47,328 Da
ncbi pathways :
ALK1 Signaling Events Pathway (137968); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Cell Cycle Pathway (83054); Cell Cycle Pathway (463); Chagas Disease (American Trypanosomiasis) Pathway (147809); Chagas Disease (American Trypanosomiasis) Pathway (147795); Chronic Myeloid Leukemia Pathway (83116); Chronic Myeloid Leukemia Pathway (528); Colorectal Cancer Pathway (83106)
ncbi summary :
This gene encodes a member of the TGF-beta family of proteins. The encoded protein is secreted and is involved in embryogenesis and cell differentiation. Defects in this gene are a cause of familial arrhythmogenic right ventricular dysplasia 1. [provided by RefSeq, Mar 2009]
uniprot summary :
Function: Involved in embryogenesis and cell differentiation.
size4 :
10x96-Strip-Wells