catalog number :
MBS703114
products type :
ELISA Kit
products full name :
Rabbit Interleukin 17, IL-17 ELISA Kit
products short name :
Interleukin 17, IL-17
products name syn :
Rabbit Interleukin 17; IL-17 ELISA Kit; Interleukin 17; IL-17
other names :
interleukin-17, partial; Interleukin-17; Interleukin-17
products gene name :
IL-17
uniprot entry name :
Q5QEX9_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of rabbit IL-17. No significant cross-reactivity or interference between rabbit IL-17 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma and tissue homogenates. Assay Type: Sandwich. Detection Range: 31.25 pg/ml -2000 pg/ml. Sensitivity: The minimum detectable dose of rabbit IL-17 is typically less than 7.81 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL-17 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-17 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-17 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-17 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
AAV41220.1
ncbi summary :
The protein encoded by this gene is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. [provided by RefSeq, Jul 2008]
uniprot summary :
IL17A: Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of ICAM1/intracellular adhesion molecule 1 in fibroblasts. Belongs to the IL-17 family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 6p12. Cellular Component: extracellular space; external side of plasma membrane. Molecular Function: cytokine activity. Biological Process: cell death; cell-cell signaling; apoptosis; positive regulation of interleukin-23 production; positive regulation of osteoclast differentiation; protein amino acid glycosylation; immune response; positive regulation of transcription from RNA polymerase II promoter; inflammatory response
size4 :
10x96-Strip-Wells