catalog number :
MBS703110
products type :
ELISA Kit
products full name :
Human Thyroid Stimulatin Hormone Receptor, TSHR ELISA Kit
products short name :
thyroid stimulating hormone receptor
products name syn :
Human Thyroid Stimulatin Hormone Receptor (TSHR) ELISA Kit; CHNG1; LGR3; MGC75129; hTSHR-I; seven transmembrane helix receptor; thyroid stimulating hormone receptor; isoform 2; thyrotropin receptor; thyrotropin receptor-I; hTSHR-I; thyroid stimulating hormone receptor
other names :
thyrotropin receptor; Thyrotropin receptor; thyrotropin receptor; TSH-R; thyroid-stimulating hormone receptor; thyroid stimulating hormone receptor<; Thyroid-stimulating hormone receptor; TSH-R
products gene name :
TSHR
other gene names :
TSHR; TSHR; TSH-R
uniprot entry name :
TSHR_BOVIN
specificity :
This assay has high sensitivity and excellent specificity for detection of human TSHR. No significant cross-reactivity or interference between human TSHR and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.5 ng/ml - 20 ng/ml. Sensitivity: The minimum detectable dose of human TSHR is typically less than 0.25 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest human TSHR concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TSHR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated antibody specific for TSHR. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TSHR bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_776631.1
ncbi gb acc num :
NM_174206.3
ncbi mol weight :
86,431 Da
ncbi pathways :
Autoimmune Thyroid Disease Pathway (84356); Autoimmune Thyroid Disease Pathway (533); Class A/1 (Rhodopsin-like Receptors) Pathway (1008554); G Alpha (s) Signalling Events Pathway (1008583); GPCR Downstream Signaling Pathway (1008582); GPCR Ligand Binding Pathway (1008553); Hormone Ligand-binding Receptors Pathway (1008567); Neuroactive Ligand-receptor Interaction Pathway (84292); Neuroactive Ligand-receptor Interaction Pathway (462); Peptide GPCRs Pathway (920907)
uniprot summary :
Function: Receptor for thyrothropin. Plays a central role in controlling thyroid cell metabolism. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Also acts as a receptor for thyrostimulin (GPA2+GPB5).
size4 :
10x96-Strip-Wells