ELISA/assay

Human Desert hedgehog, DHH ELISA Kit

MBS703098

48-Strip-Wells

565 USD

ELISA Kit

Human Desert hedgehog, DHH ELISA Kit

desert hedgehog homolog (Drosophila)

Human Desert hedgehog (DHH) ELISA kit; HHG-3; MGC35145; desert hedgehog; desert hedgehog homolog (Drosophila)

desert hedgehog protein preproprotein; Desert hedgehog protein; desert hedgehog protein; mutant desert hedgehog; desert hedgehog homolog; desert hedgehog; HHG-3Cleaved into the following 2 chains:Desert hedgehog protein N-product; Desert hedgehog protein C-product

DHH

DHH; DHH; GDXYM; HHG-3; SRXY7; DHH

DHH_HUMAN

Human

396

This assay has high sensitivity and excellent specificity for detection of human DHH. No significant cross-reactivity or interference between human DHH and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, cell culture supernates and tissue homogenates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml -1000 pg/ml. Sensitivity: 3.9 pg/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for DHH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DHH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DHH is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DHH bound in the initial step. The color development is stopped and the intensity of the color is measured.

19482158

NP_066382.1

NM_021044.2

O43323

43,577 Da

Class B/2 (Secretin Family Receptors) Pathway (106378); Disease Pathway (530764); GPCR Ligand Binding Pathway (161020); Hedgehog Signaling Pathway (198835); Hedgehog Ligand Biogenesis Pathway (1016168); Hedgehog Signaling Pathway (83063); Hedgehog Signaling Pathway (474); Hh Ligand Biogenesis Disease Pathway (1016213); Processing-defective Hh Variants Abrogate Ligand Secretion Pathway (1016212); Release Of Hh-Np From The Secreting Cell Pathway (1016169)

This gene encodes a member of the hedgehog family. The hedgehog gene family encodes signaling molecules that play an important role in regulating morphogenesis. This protein is predicted to be made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the organism. Defects in this protein have been associated with partial gonadal dysgenesis (PGD) accompanied by minifascicular polyneuropathy. This protein may be involved in both male gonadal differentiation and perineurial development. [provided by RefSeq, May 2010]

Function: Intercellular signal essential for a variety of patterning events during development. May function as a spermatocyte survival factor in the testes. Essential for testes development.

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150