ELISA/assay

Dog Matrix metalloproteinase 3, MMP-3 ELISA Kit

MBS703093

48-Strip-Wells

360 USD

ELISA Kit

Dog Matrix metalloproteinase 3, MMP-3 ELISA Kit

matrix metallopeptidase 3 (stromelysin 1, progelatinase)

Dog Matrix metalloproteinase 3; MMP-3 ELISA kit; CHDS6; MGC126102; MGC126103; MGC126104; MMP-3; SL-1; STMY; STMY1; STR1; matrix metalloproteinase 3; matrix metalloproteinase 3 (stromelysin 1; progelatinase) ; proteoglycanase; transin-1; matrix metallopeptidase 3 (stromelysin 1; progelatinase)

stromelysin-1; Stromelysin-1; stromelysin-1; SL-1; MMP-3; stromelysin 1; matrix metalloproteinase-3; Matrix metalloproteinase-3; MMP-3

MMP3

MMP3; MMP3; SL-1; MMP-3

MMP3_CANFA

Dog

478

This assay has high sensitivity and excellent specificity for detection of dog MMP-3. No significant cross-reactivity or interference between dog MMP-3 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, cell culture supernates, urine, tissue homogenates. Assay Type: Sandwich. Detection Range: 1.56 ng/ml -100 ng/ml. Sensitivity: The minimum detectable dose of dog MMP-3 is typically less than 0.39 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MMP-3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MMP-3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP-3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP-3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

50950195

NP_001002967.1

NM_001002967.1

Q6Y4Q5

53,633 Da

Activation Of Matrix Metalloproteinases Pathway (1016248); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (1016237); Collagen Degradation Pathway (1016249); Collagen Formation Pathway (1016235); Degradation Of The Extracellular Matrix Pathway (1016247); EGFR Transactivation By Gastrin Pathway (1017238); Extracellular Matrix Organization Pathway (1016234); Gastrin-CREB Signalling Pathway Via PKC And MAPK (1017237); Matrix Metalloproteinases Pathway (198594); Rheumatoid Arthritis Pathway (200312)

Function: Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase . Catalytic activity: Preferential cleavage where P1', P2' and P3' are hydrophobic residues. Cofactor: Binds 4 calcium ions per subunit

48-Strip-Wells

360 USD

96-Strip-Wells

505

5x96-Strip-Wells

1935

10x96-Strip-Wells

3595