Human Thymidine Kinase 1, Soluble, TK1 ELISA Kit | MyBioSource MBS703089 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Thymidine Kinase 1, Soluble, TK1 ELISA Kit

catalog :

MBS703089

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS703089

products type :

ELISA Kit

products full name :

Human Thymidine Kinase 1, Soluble, TK1 ELISA Kit

products short name :

thymidine kinase 1, soluble

products name syn :

Human Thymidine Kinase 1; Soluble (TK1) ELISA Kit; TK2; thymidine kinase 1; thymidine kinase 1 soluble isoform; thymidine kinase-1; thymidine kinase 1; soluble

other names :

thymidine kinase, cytosolic; Thymidine kinase, cytosolic; thymidine kinase, cytosolic; thymidine kinase-1; thymidine kinase 1 soluble isoform; thymidine kinase 1, soluble

products gene name :

TK1

other gene names :

TK1; TK1; TK2

uniprot entry name :

KITH_HUMAN

reactivity :

Human

sequence length :

234

specificity :

This assay has high sensitivity and excellent specificity for detection of Human TK1. No significant cross-reactivity or interference between Human TK1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 62.5 pg/ml-4000 pg/ml. Sensitivity: 15.6 pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Transcription/Transcription regulation. Protein Biological Process 3: DNA synthesis

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TK1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

164698438

ncbi acc num :

NP_003249.3

ncbi gb acc num :

NM_003258.4

uniprot acc num :

P04183

ncbi mol weight :

25,469 Da

ncbi pathways :

Drug Metabolism - Other Enzymes Pathway (83033); Drug Metabolism - Other Enzymes Pathway (428); E2F Transcription Factor Network Pathway (137934); Fluoropyrimidine Activity Pathway (198778); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Nucleotides Pathway (106263); Pyrimidine Metabolism Pathway (106281); Pyrimidine Metabolism Pathway (82946); Pyrimidine Metabolism Pathway (309)

uniprot summary :

TK: cytosolic thymidine kinase. Phosphorylated during mitosis. Its enzymatic activity is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells. Protein type: Xenobiotic Metabolism - drug metabolism - other enzymes; EC 2.7.1.21; Nucleotide Metabolism - pyrimidine; Kinase, other. Chromosomal Location of Human Ortholog: 17q23.2-q25.3. Cellular Component: cytosol. Molecular Function: identical protein binding; nucleoside kinase activity; zinc ion binding; thymidine kinase activity; ATP binding. Biological Process: response to nutrient levels; skeletal muscle cell proliferation; nucleobase, nucleoside and nucleotide metabolic process; response to toxin; liver development; deoxyribonucleoside monophosphate biosynthetic process; fetal process involved in parturition; protein homotetramerization; response to cortisol stimulus; pyrimidine base metabolic process; response to copper ion; gut development; nucleotide biosynthetic process; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; thymidine metabolic process; pyrimidine nucleoside salvage; phosphorylation

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage