ELISA/assay

Mouse myeloid differentiation factor 88, MyD88 ELISA Kit

MBS703085

48-Strip-Wells

590 USD

ELISA Kit

Mouse myeloid differentiation factor 88, MyD88 ELISA Kit

myeloid differentiation primary response gene (88)

Mouse myeloid differentiation factor 88; MyD88 ELISA Kit; MYD88D; myeloid differentiation primary response gene 88; myeloid differentiation primary response gene (88)

myeloid differentiation primary response protein MyD88; Myeloid differentiation primary response protein MyD88; myeloid differentiation primary response protein MyD88; myeloid differentiation primary response gene 88

MYD88

Myd88; Myd88

MYD88_MOUSE

Mouse

296

This assay has high sensitivity and excellent specificity for detection of mouse MyD88. No significant cross-reactivity or interference between mouse MyD88 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 47 pg/ml -3000 pg/ml. Sensitivity: The minimum detectable dose of mouse MyD88 is typically less than 11.7 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MyD88 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MyD88 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MyD88 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MyD88 bound in the initial step. The color development is stopped and the intensity of the color is measured.

6754772

NP_034981.1

NM_010851.2

P22366

33,753 Da

Activated TLR4 Signalling Pathway (970703); African Trypanosomiasis Pathway (194387); African Trypanosomiasis Pathway (194323); Apoptosis Pathway (83257); Apoptosis Pathway (470); Apoptosis Signaling Pathway (522973); Chagas Disease (American Trypanosomiasis) Pathway (147810); Chagas Disease (American Trypanosomiasis) Pathway (147795); Cytokine Signaling In Immune System Pathway (970814); Cytosolic Sensors Of Pathogen-associated DNA Pathway (1001263)

Function: Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway. Isoform 2 is defective in its ability to induce IRAK phosphorylation and NF-kappa-B activation and can function as a negative regulator of activation by IL-1 or lipopolysaccharide (LPS). Activates IRF1 resulting in its rapid migration into the nucleus to mediate an efficient induction of IFN-beta, NOS2/INOS, and IL12A genes. MyD88-mediated signaling in intestinal epithelial cells is crucial for maintenance of gut homeostasis and controls the expression of the antimicrobial lectin REG3G in the small intestine.8 PublicationsManual assertion based on experiment in:Ref.4

48-Strip-Wells

590 USD

96-Strip-Wells

850

5x96-Strip-Wells

2880

10x96-Strip-Wells

5440