Chicken Interleukin 1beta, IL-1beta ELISA Kit | MyBioSource MBS703082 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Chicken Interleukin 1beta, IL-1beta ELISA Kit

catalog :

MBS703082

quantity :

48-Strip-Wells

price :

490 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS703082

products type :

ELISA Kit

products full name :

Chicken Interleukin 1beta, IL-1beta ELISA Kit

products short name :

Interleukin 1beta, IL-1beta

products name syn :

Chicken Interleukin 1beta; IL-1beta ELISA Kit; Interleukin 1beta; IL-1beta

other names :

interleukin-1 beta proprotein; Interleukin-1 beta; interleukin-1 beta; IL-1 beta; catabolin; preinterleukin 1 beta; pro-interleukin-1-beta; interleukin 1, beta; Catabolin

products gene name :

IL-1beta

other gene names :

IL1B; IL1B; IL-1; IL1F2; IL1-BETA; IL1F2; IL-1 beta

uniprot entry name :

IL1B_HUMAN

reactivity :

Chicken

sequence length :

269

specificity :

This assay has high sensitivity and excellent specificity for detection of chicken IL-1beta. No significant cross-reactivity or interference between chicken IL-1beta and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Competitive. Detection Range: 0. 27 pg/ml -200 pg/ml. Sensitivity: The minimum detectable dose of chicken IL-1beta is typically less than 0.27 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest chicken IL-1beta concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with IL-1beta. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody specific to IL-1beta. The competitive inhibition reaction is launched between with pre-coated IL-1beta and IL-1beta in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of IL-1beta in the sample. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

10835145

ncbi acc num :

NP_000567.1

ncbi gb acc num :

NM_000576.2

uniprot acc num :

P01584

ncbi mol weight :

30,748 Da

ncbi pathways :

African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Alzheimers Disease Pathway (672448); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Apoptosis Pathway (83060); Apoptosis Pathway (470); Cellular Roles Of Anthrax Toxin Pathway (138076)

ncbi summary :

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.1 PublicationManual assertion based on experiment in:Ref.15

size1 :

48-Strip-Wells

price1 :

490 USD

size2 :

96-Strip-Wells

price2 :

690

size3 :

5x96-Strip-Wells

price3 :

2560

size4 :

10x96-Strip-Wells

price4 :

4790

more info or order :

MyBioSource product webpage