ELISA/assay

Human Catalase, Cat ELISA Kit

MBS703074

24-Strip-Wells (LIMIT 1)

240 USD

ELISA Kit

Human Catalase, Cat ELISA Kit

[catalase]

[Human Catalase (CAT) ELISA kit; MGC138422; MGC138424; ; catalase]

[catalase; Catalase; catalase; catalase]

[CAT]

[CAT; CAT]

CATA_HUMAN

Human

527

This assay has high sensitivity and excellent specificity for detection of human CAT. No significant cross-reactivity or interference between human CAT and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma, tissue homogenates. Detection Range: 15.6 pg/ml -1000 pg/mL. Sensitivity: <3.9 pg/mL

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CAT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CAT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CAT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CAT bound in the initial step. The color development is stopped and the intensity of the color is measured.

4557014

NP_001743.1

NM_001752.3

P04040

59,756 Da

Amyotrophic Lateral Sclerosis (ALS) Pathway (920975); Amyotrophic Lateral Sclerosis (ALS) Pathway (83099); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); Cellular Responses To Stress Pathway (645258); DNA Damage Response (only ATM Dependent) Pathway (198827); Detoxification Of Reactive Oxygen Species Pathway (941400); Folate Metabolism Pathway (198833); FoxO Family Signaling Pathway (138036); FoxO Signaling Pathway (921162); Glyoxylate And Dicarboxylate Metabolism Pathway (83002)

This gene encodes catalase, a key antioxidant enzyme in the bodies defense against oxidative stress. Catalase is a heme enzyme that is present in the peroxisome of nearly all aerobic cells. Catalase converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Oxidative stress is hypothesized to play a role in the development of many chronic or late-onset diseases such as diabetes, asthma, Alzheimer's disease, systemic lupus erythematosus, rheumatoid arthritis, and cancers. Polymorphisms in this gene have been associated with decreases in catalase activity but, to date, acatalasemia is the only disease known to be caused by this gene. [provided by RefSeq, Oct 2009]

Function: Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.1 PublicationManual assertion based on experiment in:Ref.11. Catalytic activity: 2 H2O2 = O2 + 2 H2O.1 PublicationManual assertion based on experiment in:Ref.11. Cofactor: Heme group.

24-Strip-Wells (LIMIT 1)

240 USD

48-Strip-Wells

550

96-Strip-Wells

795

5x96-Strip-Wells

2890

10x96-Strip-Wells

5465