catalog number :
MBS703064
products type :
ELISA Kit
products full name :
Rat vascular cell adhesion protein 1, VCAM-1/CD106 ELISA Kit
products short name :
vascular cell adhesion protein 1 (VCAM-1/CD106)
products name syn :
Rat vascular cell adhesion protein 1 (VCAM-1/CD106) ELISA kit; CD106; DKFZp779G2333; INCAM-100; MGC99561; CD106 antigen; vascular cell adhesion protein 1 (VCAM-1/CD106)
other names :
vascular cell adhesion protein 1; Vascular cell adhesion protein 1; vascular cell adhesion protein 1; VCAM-1; V-CAM 1; vascular cell adhesion molecule 1; CD_antigen: CD106
products gene name :
VCAM-1/CD106
other gene names :
Vcam1; Vcam1; VCAM1B; Vcam-1; V-CAM 1; VCAM-1
uniprot entry name :
VCAM1_RAT
specificity :
This assay has high sensitivity and excellent specificity for detection of rat VCAM-1. No significant cross-reactivity or interference between rat VCAM-1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 15.6 ng/ml -1000 ng/ml. Sensitivity: 3.9 ng/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for VCAM-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VCAM-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VCAM-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VCAM-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_037021.1
ncbi gb acc num :
NM_012889.1
ncbi mol weight :
81,246 Da
ncbi pathways :
Adaptive Immune System Pathway (1010925); African Trypanosomiasis Pathway (194377); African Trypanosomiasis Pathway (194323); Cell Adhesion Molecules (CAMs) Pathway (83461); Cell Adhesion Molecules (CAMs) Pathway (480); Cytokine Signaling In Immune System Pathway (1011036); Extracellular Matrix Organization Pathway (1009923); HTLV-I Infection Pathway (373894); HTLV-I Infection Pathway (373889); Immune System Pathway (1010924)
ncbi summary :
binds integrin very late antigen (VLA4); plays a role in cell adhesion [RGD, Feb 2006]
uniprot summary :
Function: Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
size4 :
10x96-Strip-Wells