catalog number :
MBS703054
products type :
ELISA Kit
products full name :
Human Rotavirus antigen, RV Ag ELISA Kit
products short name :
Rotavirus antigen, RV Ag
products name syn :
Human Rotavirus antigen; RV Ag ELISA Kit; Human Rotavirus antigen; RV Ag
products gene name :
RV Ag
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Sample Type: feces. Detection Wavelength: 450 nm
other info2 :
Sample Volume: 50-100ul. Protein Biological Process 1: Infection
products description :
For Samples This immunoassay kit allows for the in vitro semi-quantitative determination of human Rotavirus antigen concentrations in feces. PRINCIPLE OF THE ASSAY The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rotavirus antigen. Standards or samples are then added to the appropriate microtiter plate wells with a HRP-conjugated antibody preparation specific for Rotavirus antigen to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain Rotavirus antigen, HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rotavirus antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.