human ADAM metallopeptidase with thrombospondin type 1 motif4, ADAMTS4 ELISA Kit | MyBioSource MBS703047 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

human ADAM metallopeptidase with thrombospondin type 1 motif4, ADAMTS4 ELISA Kit

catalog :

MBS703047

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS703047

products type :

ELISA Kit

products full name :

human ADAM metallopeptidase with thrombospondin type 1 motif4, ADAMTS4 ELISA Kit

products short name :

ADAM metallopeptidase with thrombospondin type 1 motif, 4

products name syn :

human ADAM metallopeptidase with thrombospondin type 1 motif4; ADAMTS4 ELISA Kit; ADAMTS-2; ADAMTS-4; ADMP-1; KIAA0688; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif; 4; aggrecanase-1; ADAM metallopeptidase with thrombospondin type 1 motif; 4

other names :

A disintegrin and metalloproteinase with thrombospondin motifs 4 preproprotein; A disintegrin and metalloproteinase with thrombospondin motifs 4; A disintegrin and metalloproteinase with thrombospondin motifs 4; ADAM-TS4; ADAM-TS 4; aggrecanase-1; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 4; ADAM metallopeptidase with thrombospondin type 1 motif, 4; ADMP-1; Aggrecanase-1

products gene name :

ADAMTS4

other gene names :

ADAMTS4; ADAMTS4; ADMP-1; ADAMTS-2; ADAMTS-4; KIAA0688; UNQ769/PRO1563; ADAM-TS 4; ADAM-TS4; ADAMTS-4

uniprot entry name :

ATS4_HUMAN

reactivity :

Human

sequence length :

837

specificity :

This assay has high sensitivity and excellent specificity for detection of Human ADAMTS4. No significant cross-reactivity or interference between Human ADAMTS4 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, cell culture supernates, urine, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml-1000 pg/ml. Sensitivity: 3.9 pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADAMTS4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADAMTS4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAMTS4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAMTS4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

157427675

ncbi acc num :

NP_005090.3

ncbi gb acc num :

NM_005099.4

uniprot acc num :

O75173

ncbi mol weight :

90,197 Da

ncbi pathways :

Degradation Of The Extracellular Matrix Pathway (576263); Endochondral Ossification Pathway (198812); Extracellular Matrix Organization Pathway (576262); Metabolism Of Proteins Pathway (106230); O-glycosylation Of TSR Domain-containing Proteins Pathway (980460); O-linked Glycosylation Pathway (980459); Post-translational Protein Modification Pathway (161012)

ncbi summary :

This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme encoded by this gene lacks a C-terminal TS motif. It is responsible for the degradation of aggrecan, a major proteoglycan of cartilage, and brevican, a brain-specific extracellular matrix protein. The cleavage of aggrecan and brevican suggests key roles of this enzyme in arthritic disease and in the central nervous system, potentially, in the progression of glioma. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. Could also be a critical factor in the exacerbation of neurodegeneration in Alzheimer disease. Cleaves aggrecan at the '392-Glu- -Ala-393' site. Catalytic activity: Glutamyl endopeptidase; bonds cleaved include 370-Thr-Glu-Gly-Glu- -Ala-Arg-Gly-Ser-377 in the interglobular domain of mammalian aggrecan. Cofactor: Binds 1 zinc ion per subunit.1 PublicationManual assertion based on experiment in:Ref.9

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage