Canine connective tissue growth factor, CTGF ELISA Kit | MyBioSource MBS703042 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Canine connective tissue growth factor, CTGF ELISA Kit

catalog :

MBS703042

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS703042

products type :

ELISA Kit

products full name :

Canine connective tissue growth factor, CTGF ELISA Kit

products short name :

connective tissue growth factor, CTGF

products name syn :

Canine connective tissue growth factor; CTGF ELISA Kit; connective tissue growth factor; CTGF

other names :

connective tissue growth factor; Connective tissue growth factor; connective tissue growth factor; IBP-8; IGFBP-8; CCN family member 2; IGF-binding protein 8; hypertrophic chondrocyte-specific protein 24; insulin-like growth factor-binding protein 8; connective tissue growth factor; CCN family member 2; Hypertrophic chondrocyte-specific protein 24; Insulin-like growth factor-binding protein 8; IBP-8; IGF-binding protein 8; IGFBP-8

products gene name :

CTGF

other gene names :

CTGF; CTGF; CCN2; NOV2; HCS24; IGFBP8; CCN2; HCS24; IGFBP8; IBP-8; IGF-binding protein 8; IGFBP-8

uniprot entry name :

CTGF_HUMAN

reactivity :

Dog

sequence length :

349

specificity :

This assay has high sensitivity and excellent specificity for detection of canine CTGF. No significant cross-reactivity or interference between canine CTGF and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 62.5 pg/ml -4000 pg/ml. Sensitivity: The minimum detectable dose of canine CTGF is typically less than 15.6 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CTGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CTGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CTGF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CTGF bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

4503123

ncbi acc num :

NP_001892.1

ncbi gb acc num :

NM_001901.2

uniprot acc num :

P29279

ncbi mol weight :

35,205 Da

ncbi pathways :

Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway (160977); Gene Expression Pathway (105937); Generic Transcription Pathway (105938); Hippo Signaling Pathway (749777); Hippo Signaling Pathway (750388); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); PPARA Activates Gene Expression Pathway (530749); Regulation Of Lipid Metabolism By Peroxisome Proliferator-activated Receptor Alpha (PPARalpha) Pathway (119544); TGF-beta Receptor Signaling Pathway (138024)

ncbi summary :

The protein encoded by this gene is a mitogen that is secreted by vascular endothelial cells. The encoded protein plays a role in chondrocyte proliferation and differentiation, cell adhesion in many cell types, and is related to platelet-derived growth factor. Certain polymorphisms in this gene have been linked with a higher incidence of systemic sclerosis. [provided by RefSeq, Nov 2009]

uniprot summary :

Function: Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis.2 PublicationsManual assertion based on experiment in:Ref.14

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage