catalog number :
MBS703041
products type :
ELISA Kit
products full name :
Bovine growth hormone, GH ELISA Kit
products short name :
growth hormone 1
products name syn :
Bovine growth hormone; GH ELISA Kit; GH; GH-N; GHN; IGHD1B; hGH-N; pituitary growth hormone; growth hormone 1
other names :
somatotropin; Somatotropin; somatotropin; Growth hormone
other gene names :
GH1; GH1; GH; GH
uniprot entry name :
SOMA_BOVIN
specificity :
This assay has high sensitivity and excellent specificity for detection of bovine GH. No significant cross-reactivity or interference between bovine GH and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Competitive. Detection Range: 1.25 ng/ml -50 ng/ml. Sensitivity: The minimum detectable dose of bovine GH is typically less than 1.25 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest bovine GH concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
products description :
. Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for GH and Horseradish Peroxidase (HRP) conjugated GH. The competitive inhibition reaction is launched between with HRP labeled GH and unlabeled GH with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of GH in the sample. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_851339.1
ncbi gb acc num :
NM_180996.1
ncbi mol weight :
24,558 Da
ncbi pathways :
Cytokine Signaling In Immune System Pathway (1009189); Growth Hormone Receptor Signaling Pathway (1009206); Immune System Pathway (1009079); Jak-STAT Signaling Pathway (84316); Jak-STAT Signaling Pathway (488); Metabolism Of Proteins Pathway (942868); Neuroactive Ligand-receptor Interaction Pathway (84292); Neuroactive Ligand-receptor Interaction Pathway (462); Nuclear Signaling By ERBB4 Pathway (1008527); PI3K-Akt Signaling Pathway (692236)
uniprot summary :
Function: Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
size4 :
10x96-Strip-Wells