catalog number :
MBS703026
products type :
ELISA Kit
products full name :
Bovine Leptin, LEP ELISA Kit
products short name :
leptin
products name syn :
Bovine Leptin; LEP ELISA Kit; FLJ94114; OB; OBS; leptin (murine obesity homolog) ; leptin (obesity homolog; mouse) ; obese; mouse; homolog of; obesity factor; leptin
other names :
leptin; Leptin; leptin; obese; obesity factor; leptin (obesity homolog, mouse); leptin<; Obesity factor
other gene names :
LEP; LEP; ob; OB; OBS
uniprot entry name :
LEP_BOVIN
specificity :
This assay has high sensitivity and excellent specificity for detection of bovine LEP. No significant cross-reactivity or interference between bovine LEP and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Competitive. Detection Range: 3.12 ng/ml -50 ng/ml. Sensitivity: 3.12 ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to LEP. Standards or samples are added to the appropriate microtiter plate wells with biotin-conjugated LEP. A competitive inhibition reaction is launched between LEP (Standards or samples) and biotin-conjugated LEP with the pre-coated antibody specific for LEP. The more amount of LEP in samples, the less antibody bound by biotin-conjugated LEP. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of LEP in the sample. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_776353.2
ncbi gb acc num :
NM_173928.2
ncbi mol weight :
18,716 Da
ncbi pathways :
AMPK Signaling Pathway (989258); AMPK Signaling Pathway (992181); Adipocytokine Signaling Pathway (84332); Adipocytokine Signaling Pathway (505); Adipogenesis Pathway (920913); Cytokine-cytokine Receptor Interaction Pathway (84290); Cytokine-cytokine Receptor Interaction Pathway (460); Developmental Biology Pathway (1009461); Incretin Synthesis, Secretion, And Inactivation Pathway (1009571); Jak-STAT Signaling Pathway (84316)
uniprot summary :
Function: May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.
size4 :
10x96-Strip-Wells