catalog number :
MBS703013
products type :
ELISA Kit
products full name :
Rabbit stromal cell derived factor 1, SDF-1/CXCL12 ELISA Kit
products short name :
stromal cell derived factor 1 (SDF-1/CXCL12)
products name syn :
Rabbit stromal cell derived factor 1 (SDF-1/CXCL12) ELISA kit; stromal cell derived factor 1 (SDF-1/CXCL12)
other names :
stromal cell-derived factor 1; Stromal cell derived factor 1; stromal cell-derived factor 1; chemokine ligand 12b; stromal cell derived factor 1; stromal cell-derived factor-1 beta; chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1); chemokine (C-X-C motif) ligand 12; Stromal cell derived factor 1; Stromal cell-derived factor-1 alpha
products gene name :
SDF-1/CXCL12
other gene names :
CXCL12; SDF1; SDF1
uniprot entry name :
Q6T7C0_CHICK
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Sample Type: serum, plasma, tissue homogenates. Detection Range: 0.57 ng/ml-10 ng/ml. Detection Wavelength: 450 nm. Sensitivity: 0.36 ng/ml
other info2 :
Sample Volume: 50-100ul
products description :
Introduction: SDF-1 (stromal cell-derived factor-1) is small cytokine belonging to the chemokine family that is officially designated Chemokine (C-X-C motif) ligand 12 (CXCL12). For background information on chemokines, see CXCL11 (SCYB11; MIM 604852). Stromal cell-derived factors 1-alpha and 1-beta are small cytokines that belong to the intercrine family, members of which activate leukocytes and are often induced by proinflammatory stimuli such as lipopolysaccharide, TNF, or IL1. The intercrines are characterized by the presence of 4 conserved cysteines which form 2 disulfide bonds. They can be classified into 2 subfamilies. In the CC subfamily, which includes beta chemokine, the cysteine residues are adjacent to each other. In the CXC subfamily, which includes alpha chemokine, they are separated by an intervening amino acid. The SDF1 proteins belong to the latter group. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SDF-1. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP) conjugated monoclonal antibody preparation specific for SDF-1 and incubated. Then substrate solution A and B are added to each well. Only those wells that contain SDF-1, HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of SDF-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi acc num :
NP_989841.1
ncbi gb acc num :
NM_204510.1
ncbi mol weight :
10,104 Da
ncbi pathways :
Cytokine-cytokine Receptor Interaction Pathway 119307!!Cytokine-cytokine Receptor Interaction Pathway 460!!Intestinal Immune Network For IgA Production Pathway 128762!!Intestinal Immune Network For IgA Production Pathway 128670