catalog number :
MBS703005
products type :
ELISA Kit
products full name :
Human Saliva Peroxidase, SP ELISA Kit
products short name :
Saliva Peroxidase (SP)
products name syn :
Human Saliva Peroxidase (SP) ELISA Kit; Saliva Peroxidase (SP
other names :
lactoperoxidase isoform 3 preproprotein; Lactoperoxidase; lactoperoxidase; salivary peroxidase; lactoperoxidase; Salivary peroxidase; SPO
other gene names :
LPO; LPO; SPO; SAPX; LPO; SPO
uniprot entry name :
PERL_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of human SP. No significant cross-reactivity or interference between human SP and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, salvia, tissue homogenates. Assay Type: Sandwich. Detection Range: 0. 312 U/ml -20 U/ml. Sensitivity: The minimum detectable dose of human SP is typically less than 0.078 U/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SP bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001153574.1
ncbi gb acc num :
NM_001160102.1
ncbi mol weight :
80,288 Da
ncbi pathways :
Salivary Secretion Pathway (153376); Salivary Secretion Pathway (153352)
ncbi summary :
This gene encodes an oxidoreductase secreted from salivary, mammary, and other mucosal glands that functions as a natural antibacterial agent. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]
uniprot summary :
Function: May contribute to airway host defense against infection.1 PublicationManual assertion based on experiment in:Ref.7. Catalytic activity: 2 phenolic donor + H2O2 = 2 phenoxyl radical of the donor + 2 H2O. Cofactor: Binds 1 calcium ion per heterodimer
size4 :
10x96-Strip-Wells