ELISA/assay

Human alpha2 macroglobulin, alpha2 MG ELISA Kit

MBS702999

96 Strip Wells

850 USD

ELISA Kit

Human alpha2 macroglobulin, alpha2 MG ELISA Kit

alpha-2-macroglobulin

Human alpha2 macroglobulin; alpha2 MG ELISA Kit; CPAMD5; DKFZp779B086; FWP007; S863-7; ; alpha-2-macroglobulin

alpha-2-macroglobulin; Alpha-2-macroglobulin; alpha-2-macroglobulin; alpha-2-M; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5; alpha-2-macroglobulin; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5

A2M

A2M; A2M; A2MD; CPAMD5; FWP007; S863-7; CPAMD5; FWP007; Alpha-2-M

A2MG_HUMAN

Human

1474

This assay has high sensitivity and excellent specificity for detection of Human A2M. No significant cross-reactivity or interference between Human A2M and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, tissue homogenates. Detection Range: 1.25 ug/ml-20 ug/ml. Detection Wavelength: 450 nm. Sensitivity: 1.25 ug/ml

Sample Volume: 50-100ul

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to alpha2 MG has been pre-coated onto a microplate. Standards or samples are added to the appropriate microtiter plate wells with HRP-conjugated alpha2 MG and incubated. A competitive inhibition reaction is launched between alpha2 MG (Standards or samples) and HRP-conjugated alpha2 MG with the pre-coated antibody specific for alpha2 MG. The more amount of alpha2 MG in samples, the less antibody bound by HRP-conjugated alpha2 MG. Then the substrate solutions are added to the wells, respectively. And the color develops in opposite to the amount of alpha2 MG in the sample. The color development is stopped and the intensity of the color is measured.

66932947

NP_000005.2

NM_000014.4

P01023

163,291 Da

Complement And Coagulation Cascades Pathway 198880!!Complement And Coagulation Cascades Pathway 83073!!Complement And Coagulation Cascades Pathway 484!!Degradation Of The Extracellular Matrix Pathway 576263!!Extracellular Matrix Organization Pathway 576262!!Formation Of Fibrin Clot (Clotting Cascade) Pathway 106057!!HDL-mediated Lipid Transport Pathway 106158!!Hemostasis Pathway 106028!!IL6-mediated Signaling Events Pathway 137932!!Intrinsic Pathway 106059

Alpha-2-macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many proteases, including trypsin, thrombin and collagenase. A2M is implicated in Alzheimer disease (AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. [provided by RefSeq, Jul 2008]

Function: Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.

96 Strip Wells

850 USD